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Title

The argininosuccinate lyase gene of Chlamydomonas reinhardtii: an important tool for nuclear transformation and for correlating the genetic and molecular maps of the ARG7 locus
Authors
Debuchy, Robert Charles
Purton, Saul
Rochaix, Jean-David
Published in EMBO journal. 1989, vol. 8, no. 10, p. 2803-2809
Abstract The argininosuccinate lyase (ASL) gene of Chlamydomonas reinhardtii has been cloned using four oligonucleotide probes corresponding to highly conserved regions of the ASL polypeptide sequence. The identity of the gene was confirmed by partial sequencing. It is unique, contains several introns and spans a region less than 7.8 kb that includes highly repetitive sequences. Using a particle gun, a reliable nuclear transformation system has been established by complementing three mutants deficient in ASL activity with the wild-type ASL gene. Analysis of the transformants reveals variable patterns of integration of the transforming DNA into the nuclear genome. Previous work has mapped the mutations in the mutants arg2 and arg7 to either end of the ARG7 locus 1.0 to 1.6 recombination map units apart. Our transformation results show that these two mutations are located within a region of 7.8 kb. This allows for the first correlation of the recombination map and the molecular map at the ARG7 locus and indicates a high recombination frequency in this region of the nuclear genome.
Keywords Amino Acid Sequence — Animals — Argininosuccinate Lyase/genetics — Base Sequence — Southern Blotting — Chlamydomonas/enzymology/genetics — Molecular Cloning — Genes — Humans — Lyases/genetics — Molecular Sequence Data — Mutation — Oligonucleotides — Plasmids — Rats — Nucleic Acid Repetitive Sequences — Restriction Mapping — Nucleic Acid Sequence Homology — Genetic Transcription — Genetic Transformation
Identifiers
PMID: 2583083
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Article (Published version) (1.5 MB) - document accessible for UNIGE members only Limited access to UNIGE
Structures
Faculté des sciences / Section de biologie / Département de biologie moléculaire
Faculté des sciences / Section de biologie / Département des sciences végétales
Citation
(ISO format) 		DEBUCHY, Robert Charles, PURTON, Saul, ROCHAIX, Jean-David. The argininosuccinate lyase gene of Chlamydomonas reinhardtii: an important tool for nuclear transformation and for correlating the genetic and molecular maps of the ARG7 locus. In: EMBO Journal, 1989, vol. 8, n° 10, p. 2803-2809. https://archive-ouverte.unige.ch/unige:148884

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