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Scientific article
English

Superovulation in mice alters the methylation pattern of imprinted genes in the sperm of the offspring

Published inReproductive Toxicology, vol. 28, no. 4, p. 536-541
Publication date2009
Abstract

Some steps of the assisted reproduction techniques, such as superovulation, may interfere with imprinting reprogramming. In the present study, superovulation was induced in the mouse and its possible effects on the differentially methylated domains of 2 paternally (H19 and Gtl2) and 3 maternally (Peg1, Snrpn and Peg3) imprinted genes were tested in the male offspring over 2 generations. The CpGs methylation status was analyzed by pyro- and bisulfite sequencing. In liver, skeletal muscle and tail, no effect of superovulation could be observed. In the sperm, however, a significant 6% decrease in the number of methylated CpGs of H19 and significant 2.8- and 7.0-fold increases in those of Peg1 and Snrpn, respectively were observed following superovulation. The changes were still present in the H19 and Snrpn genes of the second generation offspring. This suggests that superovulation in the mother transgenerationally affects the offspring sperm methylation pattern.

Keywords
  • Animals
  • CpG Islands
  • DNA Methylation
  • Genomic Imprinting
  • Male
  • Mice
  • Mice
  • Inbred Strains
  • Spermatozoa/metabolism
  • Superovulation/genetics
  • SnRNP Core Proteins
Funding
  • Swiss National Science Foundation - 3100A0-116021
Citation (ISO format)
STOUDER, Christelle, DEUTSCH ESCALANTE, Samuel, GIACOBINO, Ariane. Superovulation in mice alters the methylation pattern of imprinted genes in the sperm of the offspring. In: Reproductive Toxicology, 2009, vol. 28, n° 4, p. 536–541. doi: 10.1016/j.reprotox.2009.06.009
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Identifiers
ISSN of the journal0890-6238
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