Photophysics and Photochemistry of the UV Filter Kynurenine Covalently Attached to Amino Acids and to a Model Protein

Sherin, Petr; Grilj, Jakob; Kopylova, L. V.; Yanshole, V. V.; Tsentalovich, Y. P.; Vauthey, Eric

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Title		Photophysics and Photochemistry of the UV Filter Kynurenine Covalently Attached to Amino Acids and to a Model Protein
Authors		Sherin, Petr Grilj, Jakob Kopylova, L. V. Yanshole, V. V. Tsentalovich, Y. P. Vauthey, Eric
Published in		Journal of Physical Chemistry. B, Condensed Matter, Materials, Surfaces, Interfaces and Biophysical. 2010, vol. 114, no. 36, p. 11909-11919
Abstract		The photophysics and photochemistry of kynurenine (KN) covalently bound to the amino acids lysine, cysteine, and histidine, the antioxidant glutathione, and the protein lysozyme have been studied by optical spectroscopy with femto- and nanosecond time resolution. The fluorescence quantum yield of the adducts of KN to amino acids is approximately 2 times higher than that of the free KN in solution; KN attached to protein exhibits a 7-fold increase in the fluorescence quantum yield. The S1 state dynamics of KN-modified lysozyme reveals a multiphasic decay with a broad dispersion of time constants from 1 ps to 2 ns. An increase of the triplet yield of KN bound to lysozyme is also observed; the triplet state undergoes fast intramolecular decay. The obtained results reveal an increase of the photochemical activity of KN after its covalent attachment to amino acids and proteins, which may contribute to the development of oxidative stress in the human lensessthe main causative factor for the cataract onset.
Identifiers		DOI: 10.1021/jp104485k
Full text		Article - Limited access to UNIGE
Structures		Faculté des sciences / Section de chimie et biochimie / Département de chimie physique
Citation (ISO format)		SHERIN, Petr et al. Photophysics and Photochemistry of the UV Filter Kynurenine Covalently Attached to Amino Acids and to a Model Protein. In: Journal of Physical Chemistry. B, Condensed Matter, Materials, Surfaces, Interfaces and Biophysical, 2010, vol. 114, n° 36, p. 11909-11919. https://archive-ouverte.unige.ch/unige:14788