Scientific article
Open access

Algorithms to optimize multi-column chromatographic separations of proteins

Published inJournal of Chromatography. A, vol. 1637, 461838
Publication date2021

The goal of this work was to provide a technical solution for the automated optimization of multi-column systems for protein separation and fractionation. Both algorithm and a software that can be downloaded are provided. In this algorithm, the length and order of the individual column segments can be consid- ered. Various solutions are provided by the algorithm, including i) to obtain uniform peak distribution, ii) to park the different species at the inlet of the individual column segments, and iii) to elute all species as a single peak. Two representative examples are presented, showing the possibility to obtain uniform selectivity between monoclonal antibody (mAb) sub-units, and the on-column fractioning of intact mAbs.

  • Column coupling
  • On-column fractioning
  • Optimization
  • Multi-isocratic elution
  • Monoclonal antibody
  • Protein analysis
Citation (ISO format)
CODESIDO SANCHEZ, Santiago, GUILLARME, Davy, FEKETE, Szabolcs. Algorithms to optimize multi-column chromatographic separations of proteins. In: Journal of Chromatography. A, 2021, vol. 1637, p. 461838. doi: 10.1016/j.chroma.2020.461838
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Article (Published version)
ISSN of the journal0021-9673

Technical informations

Creation01/12/2021 3:05:00 PM
First validation01/12/2021 3:05:00 PM
Update time03/15/2023 11:52:10 PM
Status update03/15/2023 11:52:09 PM
Last indexation02/12/2024 1:23:01 PM
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