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Book chapter
English

UHPLC-HRMS Analysis for Steroid Profiling in Serum (Steroidomics)

ContributorsPonzetto, Federico; Boccard, Julienorcid; Nicoli, Raul; Kuuranne, Tiia; Saugy, Martial; Rudaz, Sergeorcid
Published inMetabolic Profiling: Methods and Protocols, p. 261-278
PublisherNew York, NY : Springer
Publication date2018
Abstract

The extraction and untargeted UHPLC-HRMS analysis of endogenous steroids in serum samples is described in this protocol. The employed full-scan acquisition mode provides the adequate sensitivity to highlight the main endogenous steroids present in blood, including mineralocorticoids, progestogens, and androgens. Technical aspects for both chromatography and mass spectrometry are discussed in detail, together with a proposition of setup for sample sequence and data analysis. Furthermore, general comments are given to help the assessment of data quality and system performance.

Keywords
  • Steroids
  • Extended steroid profiles
  • Steroidomics
  • UHPLC-HRMS
Affiliation entities
Research groups
Citation (ISO format)
PONZETTO, Federico et al. UHPLC-HRMS Analysis for Steroid Profiling in Serum (Steroidomics). In: Metabolic Profiling: Methods and Protocols. New York, NY : Springer, 2018. p. 261–278. doi: 10.1007/978-1-4939-7643-0_18
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Book chapter (Published version)
accessLevelRestricted
Identifiers
Additional URL for this publicationhttp://link.springer.com/10.1007/978-1-4939-7643-0_18
ISBN978-1-4939-7642-3
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1downloads

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Creation11/03/2020 5:21:00 PM
First validation11/03/2020 5:21:00 PM
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