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Title

Development and validation of an UHPLC–MS/MS method for extended serum steroid profiling in female populations

Authors
Salamin, Olivier
Saugy, Martial
Kuuranne, Tiia
Published in Bioanalysis. 2020, vol. 12, no. 11, p. 753-768
Abstract Quantitative endogenous steroid profiling in blood appears as a complementary approach to the urinary module of the World Anti-Doping Agency's Athlete Biological Passport Steroidal Module for the detection of testosterone doping. To refine this approach further, a UHPLC–MS/MS method was developed for the simultaneous determination of 14 free and 14 conjugated steroids in serum. Results: The method was validated for quantitative purposes with satisfactory results in terms of selectivity, linearity range, trueness, precision and combined uncertainty (<20%). The validated method was then applied to serum samples from both healthy women and women diagnosed with mild hyperandrogenism. Conclusion: The UHPLC–MS/MS method showed promising capability in quantifying free and conjugated steroids in serum and determining variations of their concentration/distribution within serum samples from different populations.
Keywords female populationshyperandrogenismserumsteroid profilingUHPLC–MS/MS
Identifiers
PMID: 32479744
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Structures
Research group Sciences analytiques
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(ISO format)
SALAMIN, Olivier et al. Development and validation of an UHPLC–MS/MS method for extended serum steroid profiling in female populations. In: Bioanalysis, 2020, vol. 12, n° 11, p. 753-768. doi: 10.4155/bio-2020-0046 https://archive-ouverte.unige.ch/unige:144972

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Deposited on : 2020-11-20

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