T cell immunity. Functional heterogeneity of human memory CD4⁺ T cell clones primed by pathogens or vaccines

Becattini, Simone; Latorre, Daniela; Mele, Federico; Foglierini, Mathilde; De Gregorio, Corinne; Cassotta, Antonino; Fernandez, Blanca; Kelderman, Sander; Schumacher, Ton N; Corti, Davide; Lanzavecchia, Antonio; Sallusto, Federica

doi:10.1126/science.1260668

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Title		T cell immunity. Functional heterogeneity of human memory CD4⁺ T cell clones primed by pathogens or vaccines
Authors		Becattini, Simone Latorre, Daniela Mele, Federico Foglierini, Mathilde De Gregorio, Corinne Cassotta, Antonino Fernandez, Blanca Kelderman, Sander Schumacher, Ton N Corti, Davide Lanzavecchia, Antonio Sallusto, Federica show all authors [1 - 12]
Published in		Science. 2015, vol. 347, no. 6220, p. 400-406
Abstract		Distinct types of CD4(+) T cells protect the host against different classes of pathogens. However, it is unclear whether a given pathogen induces a single type of polarized T cell. By combining antigenic stimulation and T cell receptor deep sequencing, we found that human pathogen- and vaccine-specific T helper 1 (T(H)1), T(H)2, and T(H)17 memory cells have different frequencies but comparable diversity and comprise not only clones polarized toward a single fate, but also clones whose progeny have acquired multiple fates. Single naïve T cells primed by a pathogen in vitro could also give rise to multiple fates. Our results unravel an unexpected degree of interclonal and intraclonal functional heterogeneity of the human T cell response and suggest that polarized responses result from preferential expansion rather than priming.
Keywords		Amino Acid Sequence — CD4-Positive T-Lymphocytes/immunology — Candida albicans/immunology — Cells — Cultured — Clone Cells — High-Throughput Nucleotide Sequencing — Host-Pathogen Interactions/immunology — Humans — Immunologic Memory — Lymphocyte Activation — Molecular Sequence Data — Mycobacterium tuberculosis/immunology — Receptors — Antigen — T-Cell/genetics — T-Lymphocyte Subsets/immunology — Th1 Cells/immunology — Th17 Cells/immunology — Th2 Cells/immunology — Vaccines/immunology
Identifiers		DOI: 10.1126/science.1260668 PMID: 25477212
Full text		Article (Published version) (1.9 MB) - Limited access to UNIGE
Research group		Groupe Simone Becattini (1027)
Citation (ISO format)		BECATTINI, Simone et al. T cell immunity. Functional heterogeneity of human memory CD4⁺ T cell clones primed by pathogens or vaccines. In: Science, 2015, vol. 347, n° 6220, p. 400-406. doi: 10.1126/science.1260668 https://archive-ouverte.unige.ch/unige:144849