UNIGE document Professional Article
previous document  unige:144048  next document
add to browser collection
Title

Notch coordinates periodontal ligament maturation through regulating Lamin A.

Authors
Bolton, C
Illsley, C S
Kok, W L
Walker, J V
Poetsch, A
Tredwin, C
show hidden authors show all authors [1 - 9]
Published in Journal of Dental Research. 2019, vol. 98, no. 12, p. 1357-1366
Abstract Tooth eruption is a continuous biological process with dynamic changes at cellular and tissue levels, particularly within the periodontal ligament (PDL). Occlusion completion is a significant physiological landmark of dentition establishment. However, the importance of the involvement of molecular networks engaging in occlusion establishment on the final PDL maturation is still largely unknown. In this study, using rat and mouse molar teeth and a human PDL cell line for RNAseq and proteomic analysis, we systematically screened the key molecular links in regulating PDL maturation before and after occlusion establishment. We discovered Notch, a key molecular pathway in regulating stem cell fate and differentiation, is a major player in the event. Intercepting the Notch pathway by deleting its key canonical transcriptional factor, RBP-Jkappa, using a conditional knockout strategy in the mice delayed PDL maturation. We also identified that Lamin A, a cell nuclear lamina member, is a unique marker of PDL maturation, and its expression is under the control of Notch signaling. Our study therefore provides a deep insight of how PDL maturation is regulated at the molecular level, and we expect the outcomes to be applied for a better understanding of the molecular regulation networks in physiological conditions such as tooth eruption and movement and also for periodontal diseases.
Keywords AnimalsCell LineFibroblastsHumansLamin Type A/physiologyMiceMiceInbred StrainsPeriodontal Ligament/growth & developmentProteomicsRNA-SeqRatsRatsWistarReceptorsNotch/physiologySignal Transduction
Identifiers
PMID: 31461625
Full text
Article (Published version) (2.4 MB) - document accessible for UNIGE members only Limited access to UNIGE
Supplemental data (1.9 MB) - document accessible for UNIGE members only Limited access to UNIGE
Structures
Research group Groupe Kiliaridis Stavros (médecine dentaire) (239)
Project FNS: 31003A_176131/1
Citation
(ISO format)
DENES, Balazs Jozsef et al. Notch coordinates periodontal ligament maturation through regulating Lamin A. In: Journal of Dental Research, 2019, vol. 98, n° 12, p. 1357-1366. doi: 10.1177/0022034519871448 https://archive-ouverte.unige.ch/unige:144048

49 hits

0 download

Update

Deposited on : 2020-11-03

Export document
Format :
Citation style :