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Site-specific DC surface signatures influence CD4+ T cell co-stimulation and lung-homing

Christensen, Dennis
Andersen, Peter
Published in Frontiers in immunology. 2019, vol. 10, 1650
Abstract Dendritic cells (DCs) that drain the gut and skin are known to favor the establishment of T cell populations that home to the original site of DC-antigen (Ag) encounter by providing soluble "imprinting" signals to T cells in the lymph node (LN). To study the induction of lung T cell-trafficking, we used a protein-adjuvant murine intranasal and intramuscular immunization model to compare in vivo-activated Ag+ DCs in the lung and muscle-draining LNs. Higher frequencies of Ag+ CD11b+ DCs were observed in lung-draining mediastinal LNs (MedLN) compared to muscle-draining inguinal LNs (ILN). Ag+ CD11b+ MedLN DCs were qualitatively superior at priming CD4+ T cells, which then expressed CD49a and CXCR3, and preferentially trafficked into the lung parenchyma. CD11b+ DCs from the MedLN expressed higher levels of surface podoplanin, Trem4, GL7, and the known co-stimulatory molecules CD80, CD86, and CD24. Blockade of specific MedLN DC molecules or the use of sorted DC and T cell co-cultures demonstrated that DC surface phenotype influences the ability to prime T cells that then home to the lung. Thus, the density of dLN Ag+ DCs, and DC surface molecule signatures are factors that can influence the output and differentiation of lung-homing CD4+ T cells.
Keywords AnimalsCD4-Positive T-Lymphocytes/immunologyCell Differentiation/immunologyChemotaxisLeukocyte/immunologyDendritic Cells/immunologyLung/immunologyLymph Nodes/immunologyLymphocyte Activation/immunologyMaleMiceInbred C57BL
PMID: 31396211
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Research group Centre de vaccinologie et d'immunologie néonatale (177)
European Commission: TBVAC2020
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PEJOSKI, David et al. Site-specific DC surface signatures influence CD4+ T cell co-stimulation and lung-homing. In: Frontiers in Immunology, 2019, vol. 10, p. 1650. doi: 10.3389/fimmu.2019.01650 https://archive-ouverte.unige.ch/unige:143454

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Deposited on : 2020-10-23

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