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Titles listSite-specific DC surface signatures influence CD4+ T cell co-stimulation and lung-homing
Titles listSite-specific DC surface signatures influence CD4+ T cell co-stimulation and lung-homing
Scientific Article
unige:143454 
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Site-specific DC surface signatures influence CD4+ T cell co-stimulation and lung-homing |
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| Authors | ||
| Published in | Frontiers in Immunology. 2019, vol. 10, p. 1650 | |
| Abstract | Dendritic cells (DCs) that drain the gut and skin are known to favor the establishment of T cell populations that home to the original site of DC-antigen (Ag) encounter by providing soluble "imprinting" signals to T cells in the lymph node (LN). To study the induction of lung T cell-trafficking, we used a protein-adjuvant murine intranasal and intramuscular immunization model to compare in vivo-activated Ag+ DCs in the lung and muscle-draining LNs. Higher frequencies of Ag+ CD11b+ DCs were observed in lung-draining mediastinal LNs (MedLN) compared to muscle-draining inguinal LNs (ILN). Ag+ CD11b+ MedLN DCs were qualitatively superior at priming CD4+ T cells, which then expressed CD49a and CXCR3, and preferentially trafficked into the lung parenchyma. CD11b+ DCs from the MedLN expressed higher levels of surface podoplanin, Trem4, GL7, and the known co-stimulatory molecules CD80, CD86, and CD24. Blockade of specific MedLN DC molecules or the use of sorted DC and T cell co-cultures demonstrated that DC surface phenotype influences the ability to prime T cells that then home to the lung. Thus, the density of dLN Ag+ DCs, and DC surface molecule signatures are factors that can influence the output and differentiation of lung-homing CD4+ T cells. | |
| Keywords | Animals — CD4-Positive T-Lymphocytes/immunology — Cell Differentiation/immunology — Chemotaxis — Leukocyte/immunology — Dendritic Cells/immunology — Lung/immunology — Lymph Nodes/immunology — Lymphocyte Activation/immunology — Male — Mice — Inbred C57BL | |
| Identifiers | PMID: 31396211 | |
| Full text |
Article (Published version) (2.9 MB) - Free access Supplemental data (1.4 MB) - Free access Corrigendum (232 Kb) - Free access |
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| Structures | ||
| Research group | Centre de vaccinologie et d'immunologie néonatale (177) | |
| Project | H2020: TBVAC2020 | |
| Citation (ISO format) | PEJOSKI, David et al. Site-specific DC surface signatures influence CD4+ T cell co-stimulation and lung-homing. In: Frontiers in Immunology, 2019, vol. 10, p. 1650. doi: 10.3389/fimmu.2019.01650 https://archive-ouverte.unige.ch/unige:143454 |
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