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Scientific article
Open access
English

Neonatal T follicular helper cells are lodged in a pre-T follicular helper stage favoring innate over adaptive germinal center responses

Published inFrontiers in Immunology, vol. 10, 1845
Publication date2019
Abstract

T follicular helper (Tfh) cells have emerged as a critical limiting factor for controlling the magnitude of neonatal germinal center (GC) reactions and primary vaccine antibody responses. We compared the functional attributes of neonatal and adult Tfh cells at the transcriptomic level and demonstrated that the Tfh cell program is well-initiated in neonates although the Tfh gene-expression pattern (i.e., CXCR5, IL-21, BCL6, TBK1, STAT4, ASCL2, and c-MAF) is largely underrepresented as compared to adult Tfh cells. Importantly, we identified a TH2-bias of neonatal Tfh cells, with preferential differentiation toward short-lived pre-Tfh effector cells. Remarkably, adjuvantation with CpG-ODNs redirect neonatal pre-Tfh cells toward committed GC-Tfh cells, as illustrated by increased expression of Tfh signature genes and reduced expression of TH2-related genes.

Keywords
  • Adaptive Immunity
  • Adjuvants
  • Immunologic
  • Aging/immunology
  • Animals
  • Newborn/immunology
  • Germinal Center/cytology/immunology
  • Immunity
  • Innate
  • Interleukin-13/metabolism
  • Lymphopoiesis/genetics
  • Mice
  • Inbred C57BL
  • T-Lymphocytes
  • Helper-Inducer/immunology
  • Th2 Cells/cytology/immunology
  • Transcriptome
Citation (ISO format)
MASTELIC-GAVILLET, Beatris et al. Neonatal T follicular helper cells are lodged in a pre-T follicular helper stage favoring innate over adaptive germinal center responses. In: Frontiers in Immunology, 2019, vol. 10, p. 1845. doi: 10.3389/fimmu.2019.01845
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Identifiers
ISSN of the journal1664-3224
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