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Title

Escherichia coli RNase E has a role in the decay of bacteriophage T4 mRNA

Authors
Krisch, Henry M.
Published in Genes and Development. 1990, vol. 4, no. 5, p. 873-881
Abstract Bacteriophage T4 mRNAs are markedly stabilized, both chemically and functionally, in an Escherichia coli strain deficient in the RNA-processing endonuclease RNase E. The functional stability of total T4 messages increased 6-fold; we were unable to detect a T4 message whose functional stability was not increased. There was a 4-fold increase in the chemical stability of total T4 RNA. The degree of chemical stabilization of six specific T4 mRNAs examined varied from a maximum of 28-fold to a minimum of 1.5-fold. In the RNase E-deficient strain, several minutes delay and a slower rate of progeny production led to a reduction in final phage yield of approximately 50%. Although the effect of the rne temperature-sensitive mutation could be indirect, the simplest interpretation of our results is that RNase E acts directly in the degradation of many T4 mRNAs.
Keywords Bacteriophage T4mRNA decaymRNA processingmRNA stabilityRibonuclease E
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Swiss National Science Foundation: 3.188.88
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MUDD, Elisabeth Anne, CARPOUSIS, Agamemnon James, KRISCH, Henry M. Escherichia coli RNase E has a role in the decay of bacteriophage T4 mRNA. In: Genes and Development, 1990, vol. 4, n° 5, p. 873-881. doi: 10.1101/gad.4.5.873 https://archive-ouverte.unige.ch/unige:143395

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