Article (Published version) (2.8 MB) - 
Free accessOther version: http://www.genesdev.org/cgi/doi/10.1101/gad.4.5.873
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Scientific Article
unige:143395 
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Escherichia coli RNase E has a role in the decay of bacteriophage T4 mRNA |
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| Authors | ||
| Published in | Genes and Development. 1990, vol. 4, no. 5, p. 873-881 | |
| Abstract | Bacteriophage T4 mRNAs are markedly stabilized, both chemically and functionally, in an Escherichia coli strain deficient in the RNA-processing endonuclease RNase E. The functional stability of total T4 messages increased 6-fold; we were unable to detect a T4 message whose functional stability was not increased. There was a 4-fold increase in the chemical stability of total T4 RNA. The degree of chemical stabilization of six specific T4 mRNAs examined varied from a maximum of 28-fold to a minimum of 1.5-fold. In the RNase E-deficient strain, several minutes delay and a slower rate of progeny production led to a reduction in final phage yield of approximately 50%. Although the effect of the rne temperature-sensitive mutation could be indirect, the simplest interpretation of our results is that RNase E acts directly in the degradation of many T4 mRNAs. | |
| Keywords | Bacteriophage T4 — mRNA decay — mRNA processing — mRNA stability — Ribonuclease E | |
| Identifiers | DOI: 10.1101/gad.4.5.873 | |
| Full text |
Article (Published version) (2.8 MB) - Free access Other version: http://www.genesdev.org/cgi/doi/10.1101/gad.4.5.873 |
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| Structures | ||
| Project | Swiss National Science Foundation: 3.188.88 | |
| Citation (ISO format) | MUDD, Elisabeth Anne, CARPOUSIS, Agamemnon James, KRISCH, Henry M. Escherichia coli RNase E has a role in the decay of bacteriophage T4 mRNA. In: Genes and Development, 1990, vol. 4, n° 5, p. 873-881. doi: 10.1101/gad.4.5.873 https://archive-ouverte.unige.ch/unige:143395 |
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