Scientific article

The par region of pSC101 affects plasmid copy number as well as stability

Published inMolecular Microbiology, vol. 4, no. 11, p. 1839-1846
Publication date1990

The par locus is a segment of pSC101 that has been identified as a cis-acting determinant of plasmid stability. We show that par also determines copy number and must, therefore, play a role in plasmid replication. The segregation defect, but not the copy-number reduction, of par- replication origins is completely suppressed by a short sequence from the bacteriophage lambda gene O which is present in plasmid pKO-4. Thus, replication and segregation functions are separable from each other.

  • Ampicillin Resistance/genetics
  • Base Sequence
  • Molecular cloning
  • DNA Replication
  • Escherichia coli/genetics/growth & development
  • Kinetics
  • Molecular Sequence Data
  • Plasmids
  • Restriction Mapping
  • Nucleic acid sequence homology
  • Swiss National Science Foundation - 31-25698.88
Citation (ISO format)
MANEN-COMMANDEUR, Danielle, GOEBEL, Thomas Nicolas, CARO, Lucien. The <i>par</i> region of pSC101 affects plasmid copy number as well as stability. In: Molecular Microbiology, 1990, vol. 4, n° 11, p. 1839–1846. doi: 10.1111/j.1365-2958.1990.tb02032.x
Main files (1)
Article (Published version)
ISSN of the journal0950-382X

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