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The par region of pSC101 affects plasmid copy number as well as stability

Published in Molecular Microbiology. 1990, vol. 4, no. 11, p. 1839-1846
Abstract The par locus is a segment of pSC101 that has been identified as a cis-acting determinant of plasmid stability. We show that par also determines copy number and must, therefore, play a role in plasmid replication. The segregation defect, but not the copy-number reduction, of par- replication origins is completely suppressed by a short sequence from the bacteriophage lambda gene O which is present in plasmid pKO-4. Thus, replication and segregation functions are separable from each other.
Keywords Ampicillin Resistance/geneticsBase SequenceMolecular cloningDNA ReplicationEscherichia coli/genetics/growth & developmentKineticsMolecular Sequence DataPlasmidsRestriction MappingNucleic acid sequence homology
PMID: 2082144
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Swiss National Science Foundation: 31-25698.88
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MANEN-COMMANDEUR, Danielle, GOEBEL, Thomas Nicolas, CARO, Lucien. The par region of pSC101 affects plasmid copy number as well as stability. In: Molecular Microbiology, 1990, vol. 4, n° 11, p. 1839-1846. doi: 10.1111/j.1365-2958.1990.tb02032.x https://archive-ouverte.unige.ch/unige:143392

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