en
Scientific article
English

Immunogenotyping with antigen receptor gene probes as a diagnostic tool in childhood acute lymphoblastic leukaemia

Published inEuropean Journal of Haematology, vol. 45, no. 4, p. 215-222
Publication date1990
Abstract

13 cases of childhood acute lymphoblastic leukaemia (ALL) were studied combining cell surface marker analysis with immunogenotyping by Southern blot hybridisation with a panel of antigen receptor gene probes. The immunophenotypes were unequivocal: 7 patients had B-phenotype and 6 patients T-phenotype ALL. In several patients immunogenotypes were not fully consistent with the respective phenotypes. For example, 2 B-cell precursor ALL had rearranged TCR beta chain genes and 2 T-ALL rearrangement of Ig heavy-chain genes. All cases showed clonal rearrangement or deletions within the TCR delta gene locus. TCR delta gene rearrangements might, therefore, serve as markers of clonality but not of B- or T-lineage in immature lymphoid neoplasms. We conclude that in current diagnostic practice immunogenotyping is a supplement rather than an alternative to immunophenotyping by surface marker analysis.

Keywords
  • Adolescent
  • Bacterial Proteins
  • Burkitt Lymphoma/diagnosis/genetics
  • Child
  • Preschool child
  • DNA Probes
  • Deoxyribonuclease EcoRI
  • Deoxyribonuclease HindIII
  • Site-Specific Type II Deoxyribonucleases
  • Gene Rearrangement
  • T-Lymphocyte gene rearrangement
  • Beta-Chain T-Cell Antigen Receptor Gene Rearrangement
  • Genotype
  • Humans
  • Immunoglobulin Heavy Chains/genetics
  • Immunophenotyping
  • Infant
  • Adult T-Cell Leukemia-Lymphoma/diagnosis/genetics
  • Nucleic Acid Hybridization
  • Restriction Fragment Length Polymorphism
  • T-Cell antigen receptors/genetics
Funding
  • Swiss National Science Foundation - 31.9392.88
  • Swiss National Science Foundation - 31.9141.87
Citation (ISO format)
FEY, Martin F. et al. Immunogenotyping with antigen receptor gene probes as a diagnostic tool in childhood acute lymphoblastic leukaemia. In: European Journal of Haematology, 1990, vol. 45, n° 4, p. 215–222. doi: 10.1111/j.1600-0609.1990.tb00460.x
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Article (Published version)
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Identifiers
ISSN of the journal0902-4441
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