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Scientific article
Open access
English

The polymorphism of Hydra microsatellite sequences provides strain-specific signatures

Published inPLOS ONE, vol. 15, no. 9, e0230547
Publication date2020
Abstract

Hydra are freshwater polyps widely studied for their amazing regenerative capacity, adult stem cell populations, low senescence and value as ecotoxicological marker. Many wild-type strains of H. vulgaris have been collected worldwide and maintained effectively under laboratory conditions by asexual reproduction, while stable transgenic lines have been continuously produced since 2006. Efforts are now needed to ensure the genetic characterization of all these strains, which despite similar morphologies, show significant variability in their response to gene expression silencing procedures, pharmacological treatments or environmental conditions. Here, we established a rapid and reliable procedure at the single polyp level to produce via PCR amplification of three distinct microsatellite sequences molecular signatures that distinguish between Hydra strains and species. The TG-rich region of an uncharacterized gene (ms-c25145) helps to distinguish between Eurasian H. vulgaris-Pallas strains (Hm-105, Basel1, Basel2 and reg-16), between Eurasian and North American H. vulgaris strains (H. carnea, AEP), and between the H. vulgaris and H. oligactis species. The AT-rich microsatellite sequences located in the AIP gene (Aryl Hydrocarbon Receptor Interaction Protein, ms-AIP) also differ between Eurasian and North American H. vulgaris strains. Finally, the AT-rich microsatellite located in the Myb-Like cyclin D-binding transcription factor1 gene (ms-DMTF1) gene helps to distinguish certain transgenic AEP lines. This study shows that the analysis of microsatellite sequences, which is capable of tracing genomic variations between closely related lineages of Hydra, provides a sensitive and robust tool for characterizing the Hydra strains.

Keywords
  • Eurasian and North american Hydra strains
  • Hydra species
  • Molecular signatures
  • Microsatellite sequences
  • Genetic characterisation
  • Genomic amplification
  • Genomic variation barcoding
  • DMTF1
  • Aryl Hydrocarbon Receptor Interaction Protein
Research group
Citation (ISO format)
SCHENKELAARS, Quentin et al. The polymorphism of Hydra microsatellite sequences provides strain-specific signatures. In: PLOS ONE, 2020, vol. 15, n° 9, p. e0230547. doi: 10.1371/journal.pone.0230547
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ISSN of the journal1932-6203
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