en
Scientific article
Open access
English

Load Adaptation of Lamellipodial Actin Networks

Published inCell, vol. 171, no. 1, p. 188-200.e16
Publication date2017
Abstract

Actin filaments polymerizing against membranes power endocytosis, vesicular traffic, and cell motility. In vitro reconstitution studies suggest that the structure and the dynamics of actin networks respond to mechanical forces. We demonstrate that lamellipodial actin of migrating cells responds to mechanical load when membrane tension is modulated. In a steady state, migrating cell filaments assume the canonical dendritic geometry, defined by Arp2/3-generated 70° branch points. Increased tension triggers a dense network with a broadened range of angles, whereas decreased tension causes a shift to a sparse configuration dominated by filaments growing perpendicularly to the plasma membrane. We show that these responses emerge from the geometry of branched actin: when load per filament decreases, elongation speed increases and perpendicular filaments gradually outcompete others because they polymerize the shortest distance to the membrane, where they are protected from capping. This network-intrinsic geometrical adaptation mechanism tunes protrusive force in response to mechanical load.

Keywords
  • Cytoskeleton
  • Actin network
  • Actin dynamics
  • Cell migration
  • Membrane tension
  • Keratocyte
  • Lamellipodium
  • Correlated electron tomography
  • Cell mechanics
Citation (ISO format)
MUELLER, Jan Felix et al. Load Adaptation of Lamellipodial Actin Networks. In: Cell, 2017, vol. 171, n° 1, p. 188–200.e16. doi: 10.1016/j.cell.2017.07.051
Main files (1)
Article (Published version)
accessLevelPublic
Identifiers
ISSN of the journal0092-8674
327views
285downloads

Technical informations

Creation09/28/2020 10:00:00 AM
First validation09/28/2020 10:00:00 AM
Update time03/15/2023 10:41:19 PM
Status update03/15/2023 10:41:18 PM
Last indexation01/17/2024 11:00:15 AM
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack