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Scientific Article
unige:142032 
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SWATH data independent acquisition mass spectrometry for metabolomics |
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| Published in | Trends in Analytical Chemistry. 2019, vol. 120, no. 115278 | |
| Abstract | Systems Biology and ‘Omics' require reproducible identification and quantitation of many compounds, preferably in large sample cohorts. Liquid chromatography-mass spectrometry is important since data generated can be used for structure elucidation and highly specific targeted quantitation. Despite great success, the technique has limitations such as: compound coverage in one analysis, method development time and single sample analysis time which determines throughput. New instrument capabilities have led to improved methods, including ‘Data Independent Acquisition' so-called because acquisition is not changed by acquired data. SWATH-MS is a specific example that has quickly become prominent in proteomics because of increased peptide coverage, high quantitation accuracy, excellent reproducibility and the generation of a ‘digital map'. These capabilities are important in small molecules analyses although uptake in these applications has been slower. We describe the SWATH-MS technique, review its use in applications such as metabolomics and forensics, and summarize on-going improvements and future prospects. | |
| Keywords | LC-MS — Data independent analysis — SWATH — Metabolomics — Forensic — QUAL-QUANT | |
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| Full text |
Article (Published version) (864 Kb) - Limited access to UNIGE Other version: https://linkinghub.elsevier.com/retrieve/pii/S0165993618301961 |
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| Structures | ||
| Citation (ISO format) | BONNER, Ron, HOPFGARTNER, Gerard. SWATH data independent acquisition mass spectrometry for metabolomics. In: Trends in Analytical Chemistry, 2019, vol. 120, n° 115278. doi: 10.1016/j.trac.2018.10.014 https://archive-ouverte.unige.ch/unige:142032 |
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