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SWATH data independent acquisition mass spectrometry for metabolomics

Authors
Bonner, Ron
Published in Trends in Analytical Chemistry. 2019, vol. 120, no. 115278
Abstract Systems Biology and ‘Omics’ require reproducible identification and quantitation of many compounds, preferably in large sample cohorts. Liquid chromatography-mass spectrometry is important since data generated can be used for structure elucidation and highly specific targeted quantitation. Despite great success, the technique has limitations such as: compound coverage in one analysis, method development time and single sample analysis time which determines throughput. New instrument capabilities have led to improved methods, including ‘Data Independent Acquisition’ so-called because acquisition is not changed by acquired data. SWATH-MS is a specific example that has quickly become prominent in proteomics because of increased peptide coverage, high quantitation accuracy, excellent reproducibility and the generation of a ‘digital map’. These capabilities are important in small molecules analyses although uptake in these applications has been slower. We describe the SWATH-MS technique, review its use in applications such as metabolomics and forensics, and summarize on-going improvements and future prospects.
Keywords LC-MSData independent analysisSWATHMetabolomicsForensicQUAL-QUANT
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BONNER, Ron, HOPFGARTNER, Gerard. SWATH data independent acquisition mass spectrometry for metabolomics. In: Trends in Analytical Chemistry, 2019, vol. 120, n° 115278. doi: 10.1016/j.trac.2018.10.014 https://archive-ouverte.unige.ch/unige:142032

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Deposited on : 2020-09-28

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