Scientific article

Conservation in structure of TOC1 transposons from Chlamydomonas reinhardtii

Published inGene, vol. 104, no. 2, p. 235-239
Publication date1991

TOC1 transposons from Chlamydomonas reinhardtii have an unusual arrangement of long terminal repeats. Polymorphic regions between TOC1 transposons were identified by restriction mapping on Southern blots. The variation in size of an internal MluI fragment defines two subclasses of TOC1 elements. Full-length cloned members of each subclass of TOC1 element were compared by electron microscope heteroduplex analysis. The cloned elements were co-linear over their entire length with no large sequence discontinuities. Base substitutions and small insertion/deletion events of less than 50 bp are responsible for forming the two subclasses of TOC1 elements.

  • Restriction mapping
  • Electron microscope heteroduplexes
  • Recombinant DNA
  • Base substitutions
  • Small insertions/deletions
  • Swiss National Science Foundation - 3328086
Citation (ISO format)
DAY, Anil, ROCHAIX, Jean-David. Conservation in structure of TOC<i>1</i> transposons from <i>Chlamydomonas reinhardtii</i>. In: Gene, 1991, vol. 104, n° 2, p. 235–239. doi: 10.1016/0378-1119(91)90255-A
Main files (1)
Article (Published version)
ISSN of the journal0378-1119

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