DNA methylation influences the accessibility of DNA to transcription factors (TFs). Aberrant hypermethylation and subsequent inhibition of tumor suppressor gene (TSG) promoters is a hallmark in many cancers. Reactivation of TSGs in cancer cells by targeted demethylation of their promoters is an attractive therapeutic approach. A recently identified class of TFs, called super pioneer factors (SPFs), can bind to methylated DNA, leading to demethylation. Here, we explore the possibility of reactivation of methylated promoters of TSGs, by recruitment of SPFs via CRISPR/Cas9-mediated insertion of their binding motifs. To find additional therapeutic targets for epigenome editing, we investigated the epigenetic profile of various adipose tissues. Here, we performed methylome and transcriptome analysis on visceral adipose tissue in mice after and before treatment with cold and antibiotics.