en
Scientific article
English

Site-Specific Recombination Promoted by a Short DNA Segment of Plasmid R1 and by a Homologous Segment in the Terminus Region of the Escherichia coli Chromosome

Published inNew Biologist, vol. 3, no. 8, p. 780-788
Publication date1991
Abstract

A short DNA segment located in the kanamycin resistance region of plasmid Rl promotes site-specific recombination and plasmid maintenance. This segment has been reduced to 100 bp and subsequently to 44 bp without losing these properties. It can recombine with a similar segment located in the terminus region oftheEscherichia coli chromosome. It is proposed that this recombination is responsible for the plasmid maintenance properties of the Rl segment. The chromosomal site has been isolated; it also shows site-specific recombination activity. Sequence homologies were also found with a phage site-specific integration locus in the chromosome of Xanthomonas campestris and with the plasmid ColEl site-specific recombination locus. The recombinase required in all these systems is probably XerC, an E. coli enzyme acting on the cer site of plasmid ColEl for the conversion of plasmid dimers to monomers. It is postulated that site-specific recombination in the terminus region of the chromosome intervenes in the partitioning of the two daughter chromosomes.

Keywords
  • E. coli terminus
  • Plasmid ColEl cer site
  • Plasmid Rl
  • Site-specific recombination
  • XerC recombinase
Funding
  • Swiss National Science Foundation - 31.25698.88
Citation (ISO format)
CLERGET, Michel Olivier. Site-Specific Recombination Promoted by a Short DNA Segment of Plasmid R1 and by a Homologous Segment in the Terminus Region of the <i>Escherichia coli</i> Chromosome. In: New Biologist, 1991, vol. 3, n° 8, p. 780–788.
Main files (1)
Article (Published version)
accessLevelRestricted
Identifiers
ISSN of the journal1043-4674
155views
0downloads

Technical informations

Creation09/18/2020 2:59:00 PM
First validation09/18/2020 2:59:00 PM
Update time03/15/2023 10:35:38 PM
Status update03/15/2023 10:35:37 PM
Last indexation01/17/2024 10:51:39 AM
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack