Scientific article
Open access

Polyelectrolyte nanocomplexes based on chitosan derivatives for wound healing application

Publication date2019

Wound healing, when compromised, may be guided by biological cues such as Arg-Gly-Asp (RGD), a peptide known to induce cell adhesion and migration, eventually combined with adapted nanocarriers. Three different formulations were prepared and investigated in vitro for topical application. All formulations were based on carboxylated and trimethylated chitosan (CMTMC) displaying RGD. The polyelectrolyte nanocomplexes were prepared by mixing two oppositely charged polymers of CMTMC and chondroitin sulfate at different polymer ratios and subsequently characterized by dynamic light scattering and scanning electron microscopy. Hydrogels and foams with a high concentration of RGD-functionalized chitosan (3%) and hyaluronic acid (1.5%) that formed gel-embedded nanocomplexes were developed. In vitro assays showed absence of toxicity, ability to promote proliferation over 7 days and promotion of migration of human dermal fibroblasts treated with any of our formulations. These formulations were shown to be suitable for easy topical application and have the potential to accelerate wound healing.

  • Cell Adhesion/drug effects
  • Cell Line
  • Chitosan/administration & dosage/chemistry
  • Fibroblasts/drug effects
  • Humans
  • Hydrogels/chemistry
  • Hydrogen-Ion Concentration
  • Oligopeptides/administration & dosage/chemistry
  • Polyelectrolytes/administration & dosage/chemistry
  • Polymers/chemistry
  • Skin/drug effects
  • Wound Healing/drug effects
Research group
Citation (ISO format)
PATRULEA, Viorica et al. Polyelectrolyte nanocomplexes based on chitosan derivatives for wound healing application. In: European Journal of Pharmaceutics and Biopharmaceutics, 2019, vol. 140, p. 100–108. doi: 10.1016/j.ejpb.2019.05.009
Main files (1)
Article (Accepted version)
ISSN of the journal0939-6411

Technical informations

Creation08/25/2020 9:27:00 AM
First validation08/25/2020 9:27:00 AM
Update time03/15/2023 10:34:59 PM
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