We demonstrate an approach to fabricate a gold nanowire network that presents a macroscopic electrical conductivity based on a lipid nanotube (LNT) template with attached gold nanoparticles. The poor electrical conductivity that we have previously faced was overcome by centrifugation and resuspension of gold nanoparticle solution for removing stabilizing agents, which increased the density of gold nanoparticles on the LNTs. An additional electroless metal plating further enhanced their contacts at nanoscale. Thanks to these procedures, the sheet resistance was improved by 11 orders of magnitude. As a proof of principle, transparent conductive films were fabricated with these gold nanowires, which exhibited sheet resistance of maximum 70 Ω/□ and transmittance of 50–75% in visible light.