en
Scientific article
Open access
English

Automated incubation and digital image analysis of chromogenic media using copan WASPLab enables rapid detection of vancomycin-resistant enterococcus

Publication date2019
Abstract

Objective: The aim of the present study was to assess whether the WASPLab automation enables faster detection of vancomycin-resistant Enterococcus (VRE) on chromogenic VRE-specific plates by shortening the incubation time. Methods: Ninety different VRE culture negative rectal ESwab specimens were spiked with various concentrations (ranging from 3 × 102 to 3 × 107 CFU/ml) of 10 Enterococcus faecium strains (vancomycin MICs ranging from 32 to >256 mg/l), 3 E. faecium VanB strains (vancomycin MICs: 4, 8, and 16 mg/l), and 2 E. faecium VanB strains displaying vancomycin heteroresistance (vancomycin MICs: 64 and 96 mg/l).

Keywords
  • Copan WASPLab
  • VRE
  • Chromogenic media
  • Culture
  • Screening
  • Time to results
  • Vancomycin-resistant Enterococcus
Citation (ISO format)
CHERKAOUI, Abdessalam et al. Automated incubation and digital image analysis of chromogenic media using copan WASPLab enables rapid detection of vancomycin-resistant enterococcus. In: Frontiers in Cellular and Infection Microbiology, 2019, vol. 9, n° 379. doi: 10.3389/fcimb.2019.00379
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Article (Published version)
Identifiers
ISSN of the journal2235-2988
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