Automated incubation and digital image analysis of chromogenic media using copan WASPLab enables rapid detection of vancomycin-resistant enterococcus

Cherkaoui, Abdessalam; Renzi, Gesuele; Charretier, Yannick; Blanc, Dominique S.; Vuilleumier, Nicolas; Schrenzel, Jacques

doi:10.3389/fcimb.2019.00379

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Title		Automated incubation and digital image analysis of chromogenic media using copan WASPLab enables rapid detection of vancomycin-resistant enterococcus
Authors		Cherkaoui, Abdessalam Renzi, Gesuele Charretier, Yannick Blanc, Dominique S. Vuilleumier, Nicolas Schrenzel, Jacques
Published in		Frontiers in Cellular and Infection Microbiology. 2019, vol. 9, no. 379
Abstract		Objective: The aim of the present study was to assess whether the WASPLab automation enables faster detection of vancomycin-resistant Enterococcus (VRE) on chromogenic VRE-specific plates by shortening the incubation time. Methods: Ninety different VRE culture negative rectal ESwab specimens were spiked with various concentrations (ranging from 3 × 102 to 3 × 107 CFU/ml) of 10 Enterococcus faecium strains (vancomycin MICs ranging from 32 to >256 mg/l), 3 E. faecium VanB strains (vancomycin MICs: 4, 8, and 16 mg/l), and 2 E. faecium VanB strains displaying vancomycin heteroresistance (vancomycin MICs: 64 and 96 mg/l).
Keywords		Copan WASPLab — VRE — Chromogenic media — Culture — Screening — Time to results — Vancomycin-resistant Enterococcus
Identifiers		DOI: 10.3389/fcimb.2019.00379 PMID: 31781516
Full text		Article (Published version) (507 Kb) - Free access Other version: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6851235/
Structures		Faculté de médecine / Section de médecine clinique / Département de médecine
Research groups		Analyse génomique et fonctionnelle du staphylocoque doré (604) Mécanismes cérébraux du comportement et des fonctions cognitives (701) Chimie et protéomique clinique (270)
Citation (ISO format)		CHERKAOUI, Abdessalam et al. Automated incubation and digital image analysis of chromogenic media using copan WASPLab enables rapid detection of vancomycin-resistant enterococcus. In: Frontiers in Cellular and Infection Microbiology, 2019, vol. 9, n° 379. doi: 10.3389/fcimb.2019.00379 https://archive-ouverte.unige.ch/unige:140146