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Title

Automated incubation and digital image analysis of chromogenic media using copan WASPLab enables rapid detection of vancomycin-resistant enterococcus

Authors
Blanc, Dominique S.
Published in Frontiers in Cellular and Infection Microbiology. 2019, vol. 9, no. 379
Abstract Objective: The aim of the present study was to assess whether the WASPLab automation enables faster detection of vancomycin-resistant Enterococcus (VRE) on chromogenic VRE-specific plates by shortening the incubation time. Methods: Ninety different VRE culture negative rectal ESwab specimens were spiked with various concentrations (ranging from 3 × 102 to 3 × 107 CFU/ml) of 10 Enterococcus faecium strains (vancomycin MICs ranging from 32 to >256 mg/l), 3 E. faecium VanB strains (vancomycin MICs: 4, 8, and 16 mg/l), and 2 E. faecium VanB strains displaying vancomycin heteroresistance (vancomycin MICs: 64 and 96 mg/l).
Keywords Copan WASPLabVREChromogenic mediaCultureScreeningTime to resultsVancomycin-resistant Enterococcus
Identifiers
PMID: 31781516
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Research groups Analyse génomique et fonctionnelle du staphylocoque doré (604)
Mécanismes cérébraux du comportement et des fonctions cognitives (701)
Chimie et protéomique clinique (270)
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CHERKAOUI, Abdessalam et al. Automated incubation and digital image analysis of chromogenic media using copan WASPLab enables rapid detection of vancomycin-resistant enterococcus. In: Frontiers in Cellular and Infection Microbiology, 2019, vol. 9, n° 379. doi: 10.3389/fcimb.2019.00379 https://archive-ouverte.unige.ch/unige:140146

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Deposited on : 2020-08-24

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