Automated incubation and digital image analysis of chromogenic media using copan WASPLab enables rapid detection of vancomycin-resistant enterococcus

Cherkaoui, Abdessalam; Renzi, Gesuele; Charretier, Yannick; Blanc, Dominique S.; Vuilleumier, Nicolas; Schrenzel, Jacques

doi:10.3389/fcimb.2019.00379

Scientific article

English

Automated incubation and digital image analysis of chromogenic media using copan WASPLab enables rapid detection of vancomycin-resistant enterococcus

ContributorsCherkaoui, Abdessalam; Renzi, Gesuele; Charretier, Yannick; Blanc, Dominique S.; Vuilleumier, Nicolas; Schrenzel, Jacques

Published inFrontiers in Cellular and Infection Microbiology, vol. 9, no. 379

Publication date2019

Abstract

Objective: The aim of the present study was to assess whether the WASPLab automation enables faster detection of vancomycin-resistant Enterococcus (VRE) on chromogenic VRE-specific plates by shortening the incubation time. Methods: Ninety different VRE culture negative rectal ESwab specimens were spiked with various concentrations (ranging from 3 × 102 to 3 × 107 CFU/ml) of 10 Enterococcus faecium strains (vancomycin MICs ranging from 32 to >256 mg/l), 3 E. faecium VanB strains (vancomycin MICs: 4, 8, and 16 mg/l), and 2 E. faecium VanB strains displaying vancomycin heteroresistance (vancomycin MICs: 64 and 96 mg/l).

Keywords

Copan WASPLab
VRE
Chromogenic media
Culture
Screening
Time to results
Vancomycin-resistant Enterococcus

Affiliation entities

Faculté de médecine / Section de médecine clinique / Département de médecine

Research groups

Citation (ISO format)

CHERKAOUI, Abdessalam et al. Automated incubation and digital image analysis of chromogenic media using copan WASPLab enables rapid detection of vancomycin-resistant enterococcus. In: Frontiers in Cellular and Infection Microbiology, 2019, vol. 9, n° 379. doi: 10.3389/fcimb.2019.00379