UNIGE document Scientific Article
previous document  unige:138589  next document
add to browser collection
Title

Fluorescent Membrane Tension Probes for Super-Resolution Microscopy: Combining Mechanosensitive Cascade Switching with Dynamic-Covalent Ketone Chemistry

Authors show hidden authors show all authors [1 - 11]
Published in Journal of the American Chemical Society. 2020, vol. 142, no. 28, p. 12034-12038
Abstract We report the design, synthesis, and evaluation of fluorescent flipper probes for single-molecule super-resolution imaging of membrane tension in living cells. Reversible switching from bright-state ketones to dark-state hydrates, hemiacetals, and hemithioacetals is demonstrated for twisted and planarized mechanophores in solution and membranes. Broadband femtosecond fluorescence up-conversion spectroscopy evinces ultrafast chalcogen-bonding cascade switching in the excited state in solution. According to fluorescence lifetime imaging microscopy, the new flippers image membrane tension in live cells with record red shifts and photostability. Single-molecule localization microscopy with the new tension probes resolves membranes well below the diffraction limit.
Identifiers
Full text
Article (Published version) (2.2 MB) - document accessible for UNIGE members only Limited access to UNIGE
Other version: https://pubs.acs.org/doi/10.1021/jacs.0c04942
Structures
Research groups Groupe Matile
Groupe Vauthey
Groupe Roux
Citation
(ISO format)
GARCIA, José et al. Fluorescent Membrane Tension Probes for Super-Resolution Microscopy: Combining Mechanosensitive Cascade Switching with Dynamic-Covalent Ketone Chemistry. In: Journal of the American Chemical Society, 2020, vol. 142, n° 28, p. 12034-12038. doi: 10.1021/jacs.0c04942 https://archive-ouverte.unige.ch/unige:138589

22 hits

1 download

Update

Deposited on : 2020-07-28

Export document
Format :
Citation style :