UNIGE document Scientific Article
previous document  unige:1385  next document
add to browser collection
Title

The serpin Spn5 is essential for wing expansion in Drosophila melanogaster
Authors
Charron, Yves
Madani, Rime
Combepine, Chantal
Gajdosik, Vincent
Hwu, Yeukuang
Margaritondo, Giorgio
Vassalli, Jean-Dominique
Published in International Journal of Developmental Biology. 2008, vol. 52, no. 7, p. 933-942
Abstract Serpins, a superfamily of protease inhibitors, control proteolytic cascades in many physiological processes. Genomic studies have revealed the presence of a high number of serpin-encoding genes in Drosophila melanogaster, but their functions remain largely unknown. In a biochemical screen designed to detect protease inhibitors that may be implicated in early Drosophila development, we identified in embryos a ligand that forms a 67 kDa SDS-stable complex with the broad spectrum protease trypsin. Characterization of this ligand revealed it to be the recently described serpin, Spn5. Expression analysis by in situ and Northern blot hybridization indicated maternal transmission of the transcript as well as zygotic expression in many larval, pupal and adult tissues. Targeted repression by RNA interference did not alter early embryogenesis but resulted in a complete defect in the unfolding and expansion of the wings of freshly eclosed mutant flies, without other detectable effects on development.
Keywords Animals — Blotting, Northern — Drosophila Proteins/genetics/isolation & purification/metabolism — Drosophila melanogaster/embryology/genetics/growth & development/metabolism — Embryo, Nonmammalian — Escherichia coli/genetics — Gene Expression Regulation, Developmental — In Situ Hybridization — Plasmids — RNA Interference — Recombinant Fusion Proteins/isolation & purification/metabolism — Serpins/genetics/isolation & purification/metabolism — Wing/metabolism
Identifiers
PMID: 18956323
Full text
Article (Author postprint) (1.4 MB) - document accessible for UNIGE members only Limited access to UNIGE
Structures
Faculté de médecine / Section de médecine fondamentale / Département de médecine génétique et développement
Citation
(ISO format) 		CHARRON, Yves et al. The serpin Spn5 is essential for wing expansion in Drosophila melanogaster. In: International Journal of Developmental Biology, 2008, vol. 52, n° 7, p. 933-942. https://archive-ouverte.unige.ch/unige:1385

261 hits

0 download

Contact an author

Update request

Update

Deposited on : 2009-04-27

Export document
Format :
Citation style :