WRKY33-PIF4 loop is required for the regulation of H<sub>2</sub>O<sub>2</sub> homeostasis

Sun, Yijing; Liu, Zhixin; Guo, Jinggong; Zhu, Zhinan; Zhou, Yaping; Guo, Chenxi; Hu, Yunhe; Li, Jiaoai; Shangguan, Yan; Li, Tao; Hu, Yongjian; Wu, Rui; Li, Weiqiang; Rochaix, Jean-David; Miao, Yuchen; Sun, Xuwu

doi:10.1016/j.bbrc.2020.05.041

Scientific article

English

WRKY33-PIF4 loop is required for the regulation of H₂O₂ homeostasis

Contributors

Sun, Yijing; Liu, Zhixin; Guo, Jinggong; Zhu, Zhinan; Zhou, Yaping; Guo, Chenxi; Hu, Yunhe; Li, Jiaoai; Shangguan, Yan; Li, Tao; Hu, Yongjian; Wu, Rui; Li, Weiqiang; Rochaix, Jean-David; Miao, Yuchen; Sun, Xuwu

Published in

Biochemical and Biophysical Research Communications, vol. 527, no. 4, p. 922-928

Publication date

2020

Abstract

The reactive oxygen species (ROS) are continuously produced and are essential for mediating the growth and development of plants. However too much accumulation of ROS can result in the oxidative damage to cells, especially under the adverse environmental conditions. Plants have evolved sophisticated strategies to regulate the homeostasis of H2O2. In this study, we generated transgenic Arabidopsis plants in the Ws ecotype (Ws) background in which WRKY33 is co-suppressed (csWRKY33/Ws). Compared with Ws, csWRKY33/Ws plants accumulate more H2O2. RNA-seq analysis indicated that in csWRKY33/Ws plants, expression of oxidative stress related genes such as ascorbate peroxidase 2 (APX2) is affected. Over-expression of APX2 can rescue the phenotype of csWRKY33/Ws, suggesting that the changes in the growth of csWRKY33/Ws is duo to the higher accumulation of H2O2. Analysis of the CHIP-seq data suggested that WRKY33 can directly regulate the expression of PIF4, vice versa. qPCR analysis also confirmed that the mutual regulation between WRKY33 and PIF4. Similar to that of csWRKY33/Ws, and the accumulation of H2O2 in pif4 also increased. Taken together, our results reveal a WRKY33-PIF4 regulatory loop that appears to play an important role in regulating the growth and development of seedlings by mediating H2O2 homeostasis.

Keywords

WRKY33
PIF4
Gene expression
Regulatory loop
Reactive oxygen species
Homeostasis

UNIGE affiliation entities

Citation (ISO format)

SUN, Yijing et al. WRKY33-PIF4 loop is required for the regulation of H₂O₂ homeostasis. In: Biochemical and Biophysical Research Communications, 2020, vol. 527, n° 4, p. 922–928. doi: 10.1016/j.bbrc.2020.05.041

Article (Published version)

Identifiers

PID : unige:138458
DOI : 10.1016/j.bbrc.2020.05.041
PMID : 32423827

Journal ISSN

0006-291X

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Creation

10/07/2020 17:40:00

First validation

10/07/2020 17:40:00

Update

15/03/2023 22:17:53

Status update

15/03/2023 22:17:51

Last indexation

31/10/2024 19:13:31

WRKY33-PIF4 loop is required for the regulation of H₂O₂ homeostasis

Contributors

Published in

Publication date

Abstract

Keywords

UNIGE affiliation entities

Citation (ISO format)

Main files (1)

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Journal ISSN

Technical informations

Creation

First validation

Update

Status update

Last indexation

WRKY33-PIF4 loop is required for the regulation of H2O2 homeostasis

Contributors

Published in

Publication date

Abstract

Keywords

UNIGE affiliation entities

Citation (ISO format)

Main files (1)

Identifiers

Journal ISSN

Technical informations

Creation

First validation

Update

Status update

Last indexation

WRKY33-PIF4 loop is required for the regulation of H₂O₂ homeostasis