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Scientific article
English

Isolated small intestinal segments support auxiliary livers with maintenance of hepatic functions

Contributors

Joseph, Brigid; Berishvili Berney, Ekaterine; Benten, Daniel; Kumaran, Vinay; Liponava, Ekaterine; Bhargava, Kuldeep; Palestro, Christopher; Kakabadze, Zurab; Gupta, Sanjeev

Published in

Nature Medicine, vol. 10, no. 7, p. 749-753

Publication date

2004

Abstract

We determine here the functional integrity of auxiliary livers in containers fashioned from the small intestine. Liver microfragments from dipeptidyl peptidase 4 (DPP4)-deficient rats were transplanted into syngeneic normal animals with isolated intestinal segments characterized by mucosal denudation but intact vascular supply. Transplanted liver fragments were restored to confluent tissue with normal hepatic architecture and development of DPP4-positive vessels, indicating angiogenesis and revascularization. Auxiliary liver units expressed multiple hepatotrophic and angiogenic genes, and transplanted tissues remained intact for up to the 6-week duration of the studies with neither ischemic injury nor significant hepatocellular proliferation. Hepatic metabolic, transport and synthetic functions were preserved in auxiliary livers, including uptake and biliary excretion of (99m)Tc-mebrofenin in syngeneic recipients of liver from F344 rats, as well as secretion of albumin in allografted Nagase analbuminemic rats. This ability to produce functionally competent auxiliary livers in vascularized intestinal segments offers therapeutic potential for liver disease and genetic deficiency.

Keywords

  • Animals
  • Cell Communication
  • Dipeptidyl Peptidase 4/analysis
  • Gene Expression Regulation
  • Intestine, Small/blood supply/physiology
  • Liver/physiology
  • Liver Transplantation
  • Rats
  • Rats, Inbred F344
  • Rats, Long-Evans

UNIGE affiliation entities

Not a UNIGE publication

UNIGE research groups

Citation (ISO format)

JOSEPH, Brigid et al. Isolated small intestinal segments support auxiliary livers with maintenance of hepatic functions. In: Nature Medicine, 2004, vol. 10, n° 7, p. 749–753. doi: 10.1038/nm1057

Main files (1)

Article (Published version)

Identifiers

Additional URL for this publication

https://www.nature.com/articles/nm1057

Journal ISSN

1078-8956
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Technical informations

Creation

03/07/2020 14:03:00

First validation

03/07/2020 14:03:00

Update

15/03/2023 22:17:08

Status update

15/03/2023 22:17:08

Last indexation

31/10/2024 19:11:59
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