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Mapping Cell Membrane Organization and Dynamics Using Soft Nanoimprint Lithography

Sansen, T.
Sanchez-Fuentes, D.
Rathar, R.
El Alaoui, F.
Viaud, J.
de Rossi, S.
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Published in ACS applied materials & interfaces. 2020, vol. 12, no. 26, p. 29000-29012
Abstract Membrane shape is a key feature of many cellular processes, including cell differentiation, division, migration, and trafficking. The development of nanostructured surfaces allowing for the in situ manipulation of membranes in living cells is crucial to understand these processes, but this requires complicated and limited-access technologies. Here, we investigate the self-organization of cellular membranes by using a customizable and benchtop method allowing one to engineer 1D SiO2 nanopillar arrays of defined sizes and shapes on high-performance glass compatible with advanced microscopies. As a result of this original combination, we provide a mapping of the morphology-induced modulation of the cell membrane mechanics, dynamics and steady-state organization of key protein complexes implicated in cellular trafficking and signal transduction.
Keywords Cellular membranesNanostructured materialsProteinsBiointerfacesMicroscopy
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Other version: https://pubs.acs.org/doi/10.1021/acsami.0c05432
Research groups Groupe Matile
NCCR Chemical Biology
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SANSEN, T. et al. Mapping Cell Membrane Organization and Dynamics Using Soft Nanoimprint Lithography. In: ACS Applied Materials & Interfaces, 2020, vol. 12, n° 26, p. 29000-29012. doi: 10.1021/acsami.0c05432 https://archive-ouverte.unige.ch/unige:138225

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Deposited on : 2020-07-02

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