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A chloroplast gene is required for the light-independent accumulation of chlorophyll in Chlamydomonas reinhardtii

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Published in EMBO Journal. 1992, vol. 11, no. 5, p. 1697-1704
Abstract The light-independent pathway of chlorophyll synthesis which occurs in some lower plants and algae is still largely unknown. We have characterized a chloroplast mutant, H13, of Chlamydomonas reinhardtii which is unable to synthesize chlorophyll in the dark and is also photosystem I deficient. The mutant has a 2.8 kb deletion as well as other rearrangements of its chloroplast genome. By performing particle gun mediated chloroplast transformation of H13 with defined wild-type chloroplast DNA fragments, we have identified a new chloroplast gene, chlN, coding for a 545 amino acid protein which is involved in the light-independent accumulation of chlorophyll, probably at the step of reduction of protochlorophyllide to chlorophyllide. The chlN gene is also found in the chloroplast genomes of liverwort and pine, but is absent from the chloroplast genomes of tobacco and rice.
Keywords Algal ProteinsAmino Acid SequenceAnimalsBase SequenceChlamydomonas reinhardtii/genetics/metabolismChlorophyll/biosynthesis/genetics/metabolismChloroplastsChromosome DeletionDNA/geneticsLightMolecular Sequence DataMutationPlant Proteins/genetics/metabolismRNA/metabolismRestriction MappingSequence HomologyNucleic Acid
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PMID: 1374710
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Project FNS: 31.26345.89
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CHOQUET, Yves Marcel et al. A chloroplast gene is required for the light-independent accumulation of chlorophyll in Chlamydomonas reinhardtii. In: EMBO Journal, 1992, vol. 11, n° 5, p. 1697-1704. https://archive-ouverte.unige.ch/unige:138211

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