Epilepsy is a chronic neurological disorder characterized by recurrent epileptic seizures which affects 1% of the world's population, of whom 80% live in low- and middle-income countries. Antiseizure drugs (ASDs) and therapies are available for epilepsy management but despite these possibilities, a third of epileptic patients suffer from pharmaco-resistance. There is, therefore, an urgent need for new and cost-effective treatments to manage epilepsy. Herbs used in traditional medicine remain the first-line treatment for most people with limited access to ASDs. Thus, in this thesis, three plants traditionally used to treat epilepsy and CNS-related disorders were selected and studied using a bioactivity-guided isolation protocol combined to an in vivo zebrafish epilepsy model to effectively identify compounds with significant antiseizure activity. This work demonstrated the chemical diversity of antiseizure constituents and their mode of action which may help to provide evidence-based data for the management of epilepsy with well-selected medicinal herbs.