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Title

A divergent cyclin/cyclin-dependent kinase complex controls the atypical replication of a malaria parasite during gametogony and transmission
Authors
Balestra, Aurélia
Zeeshan, Mohammad
Rea, Edward
Pasquarello Mosimann, Carla
Brusini, Lorenzo
Mourier, Tobias
Subudhi, Amit Kumar
Klages Jemelin, Natacha
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Published in eLife. 2020, vol. 9, e56474
Abstract Cell cycle transitions are generally triggered by variation in the activity of cyclin-dependent kinases (CDKs) bound to cyclins. Malaria-causing parasites have a life cycle with unique cell-division cycles, and a repertoire of divergent CDKs and cyclins of poorly understood function and interdependency. We show that Plasmodium berghei CDK-related kinase 5 (CRK5), is a critical regulator of atypical mitosis in the gametogony and is required for mosquito transmission. It phosphorylates canonical CDK motifs of components in the pre-replicative complex and is essential for DNA replication. During a replicative cycle, CRK5 stably interacts with a single Plasmodium-specific cyclin (SOC2), although we obtained no evidence of SOC2 cycling by transcription, translation or degradation. Our results provide evidence that during Plasmodium male gametogony, this divergent cyclin/CDK pair fills the functional space of other eukaryotic cell-cycle kinases controlling DNA replication.
Keywords Cyclin — cell cycle — kinase — Plasmodium — transmission
Identifiers
PMID: 32568069
Full text
Article (Published version) (3.8 MB) - public document Free access
Supplemental data (6.5 MB) - public document Free access
Dataset: https://www.ebi.ac.uk/pride/archive/projects/PXD017308
Dataset: https://www.ebi.ac.uk/pride/archive/projects/PXD017283
Dataset: https://www.ebi.ac.uk/pride/archive/projects/PXD017622
Dataset: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE144743
Structures
Faculté de médecine / Section de médecine fondamentale / Département de microbiologie et médecine moléculaire
Research group Signalisation chez les parasites du paludisme (955)
Projects
Swiss National Science Foundation: BSSGI0_155852
Swiss National Science Foundation: 31003A_179321
Citation
(ISO format) 		BALESTRA, Aurélia et al. A divergent cyclin/cyclin-dependent kinase complex controls the atypical replication of a malaria parasite during gametogony and transmission. In: eLife, 2020, vol. 9, p. e56474. doi: 10.7554/eLife.56474 https://archive-ouverte.unige.ch/unige:137985

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Deposited on : 2020-06-26

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