Title |
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A divergent cyclin/cyclin-dependent kinase complex controls the atypical replication of a malaria parasite during gametogony and transmission
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Authors | | Zeeshan, Mohammad Rea, Edward Mourier, Tobias Subudhi, Amit Kumar Pandey, Rajan Brady, Declan Vaughan, Sue Holder, Anthony A Pain, Arnab Ferguson, David Jp Tewari, Rita show all authors [1 - 18]
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Published in |
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eLife. 2020, vol. 9, p. e56474 |
Abstract |
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Cell cycle transitions are generally triggered by variation in the activity of cyclin-dependent kinases (CDKs) bound to cyclins. Malaria-causing parasites have a life cycle with unique cell-division cycles, and a repertoire of divergent CDKs and cyclins of poorly understood function and interdependency. We show that Plasmodium berghei CDK-related kinase 5 (CRK5), is a critical regulator of atypical mitosis in the gametogony and is required for mosquito transmission. It phosphorylates canonical CDK motifs of components in the pre-replicative complex and is essential for DNA replication. During a replicative cycle, CRK5 stably interacts with a single Plasmodium-specific cyclin (SOC2), although we obtained no evidence of SOC2 cycling by transcription, translation or degradation. Our results provide evidence that during Plasmodium male gametogony, this divergent cyclin/CDK pair fills the functional space of other eukaryotic cell-cycle kinases controlling DNA replication. |
Keywords |
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Cyclin — cell cycle — kinase — Plasmodium — transmission |
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Research group | | Signalisation chez les parasites du paludisme (955) |
Projects | | FNS: BSSGI0_155852 FNS: 31003A_179321 |
Citation (ISO format) | | BALESTRA, Aurélia et al. A divergent cyclin/cyclin-dependent kinase complex controls the atypical replication of a malaria parasite during gametogony and transmission. In: eLife, 2020, vol. 9, p. e56474. doi: 10.7554/eLife.56474 https://archive-ouverte.unige.ch/unige:137985 |