UNIGE document Scientific Article
previous document  unige:137144  next document
add to browser collection
Title

Rapid detection of the main human plasma glycoproteins by two-dimensional polyacrylamide gel electrophoresis lectin affinoblotting

Authors
Published in Electrophoresis. 1995, vol. 16, no. 1, p. 1187-1189
Abstract Glycoprotein modifications in the glycan moiety can occur in diseases such as cancers, inflammatory processes and alcoholism. We combined high‐resolution two‐dimensional polyacrylamide gel electrophoresis (2‐D PAGE) with lectin affinoblotting in order to establish the normal human plasma glycoprotein map. Human plasma proteins were separated by mini 2‐D PAGE (7 × 9 cm), transferred onto polyvinylidene difluoride membranes and incubated with biotinylated lectins. We focused our study on lectins binding sialic acid and galactose residues. Known plasma glycoproteins such as αl‐antitrypsin, αl‐antichymotrypsin, α2‐HS glycoprotein, αl‐acid glycoprotein, haptoglobin β‐chain and transferrin were easily detected in ng amounts. This protocol was adequate to establish a normal plasma glycoprotein map and will allow the study of glycoproteins in diseases.
Keywords Two-dimensional polyacrylamide gel electrophoresisPlasma glycoproteinsLectin affinoblotting
Identifiers
PMID: 7498164
Full text
Article (Published version) (597 Kb) - document accessible for UNIGE members only Limited access to UNIGE
Other version: http://doi.wiley.com/10.1002/elps.11501601197
Structures
Citation
(ISO format)
GOLAZ, Olivier Georges et al. Rapid detection of the main human plasma glycoproteins by two-dimensional polyacrylamide gel electrophoresis lectin affinoblotting. In: Electrophoresis, 1995, vol. 16, n° 1, p. 1187-1189. doi: 10.1002/elps.11501601197 https://archive-ouverte.unige.ch/unige:137144

62 hits

0 download

Update

Deposited on : 2020-06-16

Export document
Format :
Citation style :