Desinfected seeds of Lupinus mutabilis were allowed to germinate in vitro. The seedlings were early transferred on a Murashige and Skoog medium in the presence of BAP or TDZ, which allowed axillary proliferation from the cotyledonary buds. The shoots raised were further used for continued multiplication in the presence of 10 μM BAP. Higher concentrations of BAP and TDZ induced vitrification. Before rooting, the shoots were elongated in the absence growth of regulators. Rooting was achieved through the use of two successive rooting media: an "induction" one with IBA or NAA in dark for 7 days, followed by an "expressive" medium without auxin, in light. There was a relationship between the rooting rate and the degree of shoot elongation.