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Scientific article
English

Weak expression of the pathogenesis related PR-b1 gene and localization of related protein during symbiotic endomycorrhizal interactions in tobacco roots

Published inEndocytobiosis and Cell Research, vol. 8, no. 2-3, p. 177-185
Publication date1992
Abstract

The production of pathogenesis related PR-b₁ protein has been compared in tobacco roots infected by the pathogenic fungus Chalara elegans and during symbiotic interactions with the vesicular arbuscular endomycorrhizal fungus Glomus mosseae. PR-b₁ protein could be detected by Western blotting of soluble protein extracts from pathogen-infected roots but not of endomycorrhizal tobacco roots. However, Northern analysis of total RNA extracts from endomycorrhizal roots revealed a single hybridization signal which was extremely weak when compared to that of C. elegans-infected tobacco roots. Indirect immunogold labelling showed that PR-b₁ antigen was very localized in endomycorrhizal roots and limited to host material in the interfacial matrix surrounding living intracellular fungal haustoria (arbuscules) in parenchyma cortical cells. Results are discussed in relation to PR-b₁ protein synthesis in pathogen interactions and host control in the endomycorrhizal symbiosis.

Keywords
  • Endomycorrhiza symbiosis
  • G. mosseae
  • Tobacco
  • C.elegans
  • RNA
  • PR-b₁ protein
  • Immunolocalization
Affiliation Not a UNIGE publication
Citation (ISO format)
GIANINAZZI-PEARSON, Vivienne et al. Weak expression of the pathogenesis related PR-b<sub>1</sub> gene and localization of related protein during symbiotic endomycorrhizal interactions in tobacco roots. In: Endocytobiosis and Cell Research, 1992, vol. 8, n° 2-3, p. 177–185.
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Identifiers
  • PID : unige:136953
ISSN of the journal0256-1514
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