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CCAAT/enhancer-binding protein mRNA is translated into multiple proteins with different transcription activation potentials

Published inProceedings of the National Academy of Sciences, vol. 90, no. 17, p. 8219-8223
Publication date1993
Abstract

The CCAAT/enhancer-binding protein (C/EBP) alpha is a leucine zipper protein that is preferentially expressed in certain cell types, such as adipocytes and hepatocytes. Here we show that C/EBP alpha mRNA is translated into two major proteins, C/EBP-42 and C/EBP-30, that differ in their content of N-terminal amino acid sequences. These results are best explained by a ribosome-scanning mechanism in which a fraction of ribosomes ignore the first two AUGs and initiate translation at an AUG located 351 nt downstream of the first one. Because C/EBP-30, the translation product initiated at the third AUG, is devoid of the potent transcription-activation domain contained in C/EBP-42, the former protein stimulates transcription from the mouse albumin promoter much less efficiently than the latter. The gene encoding the liver-enriched transcriptional-activator protein LAP (C/EBP-beta) has also been shown to issue two proteins, LAP and the liver-enriched transcriptional-inhibitory protein LIP, with different transcription-activation potentials. The production of multiple proteins from a single mRNA is not only shared between different C/EBP family members but also appears to be conserved in vertebrate evolution.

Keywords
  • Ribosome scanning
  • Transcription activation
  • Liver gene expression
Citation (ISO format)
OSSIPOW, Vincent, DESCOMBES, Patrick, SCHIBLER, Ulrich. CCAAT/enhancer-binding protein mRNA is translated into multiple proteins with different transcription activation potentials. In: Proceedings of the National Academy of Sciences, 1993, vol. 90, n° 17, p. 8219–8223. doi: 10.1073/pnas.90.17.8219
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ISSN of the journal0027-8424
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