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Title

Directed disruption of the Chlamydomonas chloroplast psbK gene destabilizes the photosystem II reaction center complex
Authors
Takahashi, Yuichiro
Matsumoto, Hideki
Goldschmidt-Clermont, Michel P.
Rochaix, Jean-David
Published in Plant molecular biology. 1994, vol. 24, no. 5, p. 779-788
Abstract Using particle gun-mediated chloroplast transformation we have disrupted the psbK gene of Chlamydomonas reihardtii with an aadA expression cassette that confers resistance to spectinomycin. The transformants are unable to grow photoautotrophically, but they grow normally in acetate-containing medium. They are deficient in photosystem II activity as measured by fluorescence transients and O2 evolution and they accumulate less than 10% of wild-type levels of photosystem II as measured by immunochemical means. Pulse-labeling experiments indicate that the photosystem II complex is synthesized normally in the transformants. These results differ from those obtained previously with similar cyanobacterial psbK mutants that were still capable of photoautotrophic growth (Ikeuchi et al., J. Biol. Chem. 266 (1991) 1111–1115). In C. reinhardtii the psbK product is required for the stable assembly and/or stability of the photosystem II complex and essential for photoautotrophic growth. The data also suggest that the stability requirements of the photosynthetic complexes differ considerably between C. reinhardtii and cyanobacteria.
Keywords Chlamydomonas reinhardtii — Chloroplast — Transformation — Photosystem II — psbK
Identifiers
Full text
Article (Published version) (1.2 MB) - public document Free access
Other version: http://link.springer.com/10.1007/BF00029859
Structures
Faculté des sciences / Section de biologie / Département de biologie moléculaire
Faculté des sciences / Section de biologie / Département de botanique et biologie végétale
Project
Swiss National Science Foundation: 31-34014.92
Citation
(ISO format) 		TAKAHASHI, Yuichiro et al. Directed disruption of the Chlamydomonas chloroplast psbK gene destabilizes the photosystem II reaction center complex. In: Plant Molecular Biology, 1994, vol. 24, n° 5, p. 779-788. doi: 10.1007/BF00029859 https://archive-ouverte.unige.ch/unige:135751

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