en
Scientific article
Open access
English

Complementation of a Chlamydomonas reinhardtii mutant using a genomic cosmid library

Published inPlant Molecular Biology, vol. 24, no. 3, p. 533-537
Publication date1994
Abstract

We report the rescue of an arginine-requiring mutant (arg7-8) of Chlamydomonas reinhardtii by complementation using total DNA from a genomic cosmid library. Using the glass-bead transformation method of Kindle [8] four putative transformants able to grow in the absence of exogenous arginine were obtained from 3×109 treated cells. Southern blot analysis reveals that at least three of the clones have acquired an additional copy of the gene (ARG7) encoding argininosuccinate lyase (ASL). The arginine-independent phenotype is stable in the absence of selective pressure and high levels of ASL activity are detected in all four clones. We conclude that these represent true transformants and that any stable nuclear mutant of Chlamydomonas could be rescued using this approach.

Keywords
  • Arg7 mutant
  • Chlamydomonas
  • Complementation
  • Cosmid library
Funding
  • Swiss National Science Foundation - 31.26345.89
Citation (ISO format)
PURTON, Saul, ROCHAIX, Jean-David. Complementation of a <i>Chlamydomonas reinhardtii</i> mutant using a genomic cosmid library. In: Plant Molecular Biology, 1994, vol. 24, n° 3, p. 533–537. doi: 10.1007/BF00024121
Main files (1)
Article (Published version)
Identifiers
ISSN of the journal0167-4412
210views
116downloads

Technical informations

Creation05/13/2020 4:28:00 PM
First validation05/13/2020 4:28:00 PM
Update time03/15/2023 9:55:14 PM
Status update03/15/2023 9:55:14 PM
Last indexation02/12/2024 1:12:27 PM
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack