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Title

Coupling non-denaturing chromatography to mass spectrometry for the characterization of monoclonal antibodies and related products

Authors
Farsang, Evelin
Beck, Alain
Lauber, Matthew
Schmudlach, Andrew
Published in Journal of pharmaceutical and biomedical analysis. 2020, vol. 185, 113207
Abstract The hyphenation of non-denaturing liquid chromatographic (LC) modes (ion exchange (IEX), size exclusion (SEC) and hydrophobic interaction chromatography (HIC)) with mass spectrometry (MS) has attracted significant attention in the last few years. The inherent problem of these couplings is that non-denaturing LC separations have tended to use non-volatile mobile phase additives. Indeed, classical methods have not been directly compatible with MS. Therefore two approaches can be used to address this challenge: (1) finding innovative volatile mobile phases or (2) adding a desalting step prior to MS detection via the use of multidimensional LC. These two possibilities have been applied to the characterization of charge-, size- and hydrophobic variants of various monoclonal antibodies (mAbs) and related products and have been reviewed in this paper.
Keywords Ion-exchange chromatography (IEX)Hydrophobic interaction chromatography (HIC)Size exclusion chromatography (SEC)Volatile bufferMass spectrometryMulti-Dimensional liquid chromatography
Identifiers
PMID: 32143115
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Structures
Research group Sciences analytiques
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FARSANG, Evelin et al. Coupling non-denaturing chromatography to mass spectrometry for the characterization of monoclonal antibodies and related products. In: Journal of Pharmaceutical and Biomedical Analysis, 2020, vol. 185, p. 113207. doi: 10.1016/j.jpba.2020.113207 https://archive-ouverte.unige.ch/unige:133133

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Deposited on : 2020-03-26

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