Coupling non-denaturing chromatography to mass spectrometry for the characterization of monoclonal antibodies and related products

Farsang, Evelin; Guillarme, Davy; Veuthey, Jean-Luc; Beck, Alain; Lauber, Matthew; Schmudlach, Andrew; Fekete, Szabolcs

doi:10.1016/j.jpba.2020.113207

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Title		Coupling non-denaturing chromatography to mass spectrometry for the characterization of monoclonal antibodies and related products
Authors		Farsang, Evelin Guillarme, Davy Veuthey, Jean-Luc Beck, Alain Lauber, Matthew Schmudlach, Andrew Fekete, Szabolcs
Published in		Journal of pharmaceutical and biomedical analysis. 2020, vol. 185, 113207
Abstract		The hyphenation of non-denaturing liquid chromatographic (LC) modes (ion exchange (IEX), size exclusion (SEC) and hydrophobic interaction chromatography (HIC)) with mass spectrometry (MS) has attracted significant attention in the last few years. The inherent problem of these couplings is that non-denaturing LC separations have tended to use non-volatile mobile phase additives. Indeed, classical methods have not been directly compatible with MS. Therefore two approaches can be used to address this challenge: (1) finding innovative volatile mobile phases or (2) adding a desalting step prior to MS detection via the use of multidimensional LC. These two possibilities have been applied to the characterization of charge-, size- and hydrophobic variants of various monoclonal antibodies (mAbs) and related products and have been reviewed in this paper.
Keywords		Ion-exchange chromatography (IEX) — Hydrophobic interaction chromatography (HIC) — Size exclusion chromatography (SEC) — Volatile bufferMass spectrometry — Multi-Dimensional liquid chromatography
Identifiers		DOI: 10.1016/j.jpba.2020.113207 PMID: 32143115
Full text		Article (Published version) (2.5 MB) - Limited access to UNIGE Other version: https://linkinghub.elsevier.com/retrieve/pii/S0731708519330742
Structures		Faculté des sciences / Section des sciences pharmaceutiques
Research group		Sciences analytiques
Citation (ISO format)		FARSANG, Evelin et al. Coupling non-denaturing chromatography to mass spectrometry for the characterization of monoclonal antibodies and related products. In: Journal of Pharmaceutical and Biomedical Analysis, 2020, vol. 185, p. 113207. doi: 10.1016/j.jpba.2020.113207 https://archive-ouverte.unige.ch/unige:133133