UNIGE document Scientific Article
previous document  unige:13276  next document
add to browser collection

Ibuprofen transport into and through skin from topical formulations: in vitro-in vivo comparison

Hadgraft, Jonathan
Published in Journal of investigative dermatology. 2007, vol. 127, no. 1, p. 135-42
Abstract The goal was to compare ibuprofen transport into and through skin in vivo in man and in vitro (across silicone membranes and freshly excised pig skin) from four marketed formulations. Ibuprofen gels were administered in vivo for 30 minutes. The stratum corneum (SC) at the application site was then tape-stripped, quantified gravimetrically, and extracted for drug analysis. Together with concomitant transepidermal water loss measurements, SC drug concentration-depth profiles were reproducibly determined and fitted mathematically to obtain a partition coefficient, a first-order rate constant related to ibuprofen diffusivity, and the total drug amount in the SC at the end of the application. All derived parameters were consistent across formulations. Ibuprofen permeation data through both silicone membrane and pig ear skin were also fitted to yield partitioning and diffusion parameters. The former revealed that ibuprofen partitioned differently from the gels into this model barrier. Across pig skin, however, better correlation with in vivo results was found. The dermatopharmacokinetic approach, using SC tape-stripping, offers a valid method to assess equivalency between topical drug formulations. In vitro experiments must be extrapolated cautiously to the clinic, especially when complex interactions between real formulations, which deliver both drug and excipients, and the skin occur.
Keywords Administration, TopicalAdultAnimalsBiological TransportBody Water/metabolismFemaleHumansIbuprofen/administration & dosage/pharmacokineticsMaleMembranes, ArtificialMiddle AgedPermeabilitySiliconesSkin/metabolismSwine
PMID: 16858418
Full text
Article (Published version) (218 Kb) - document accessible for UNIGE members only Limited access to UNIGE
(ISO format)
HERKENNE, Christophe et al. Ibuprofen transport into and through skin from topical formulations: in vitro-in vivo comparison. In: Journal of investigative dermatology, 2007, vol. 127, n° 1, p. 135-42. doi: 10.1038/sj.jid.5700491 https://archive-ouverte.unige.ch/unige:13276

459 hits

0 download


Deposited on : 2011-01-27

Export document
Format :
Citation style :