Coordination of Hpr1 and ubiquitin binding by the UBA domain of the mRNA export factor Mex67

Hobeika, Maria; Brockmann, Christoph; Iglesias, Nahid; Gwizdek, Carole; Neuhaus, David; Stutz, Françoise; Stewart, Murray; Divita, Gilles; Dargemont, Catherine

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Title		Coordination of Hpr1 and ubiquitin binding by the UBA domain of the mRNA export factor Mex67
Authors		Hobeika, Maria Brockmann, Christoph Iglesias, Nahid Gwizdek, Carole Neuhaus, David Stutz, Françoise Stewart, Murray Divita, Gilles Dargemont, Catherine show all authors [1 - 9]
Published in		Molecular Biology of the Cell. 2007, vol. 18, no. 7, p. 2561-8
Abstract		The ubiquitin-associated (UBA) domain of the mRNA nuclear export receptor Mex67 helps in coordinating transcription elongation and nuclear export by interacting both with ubiquitin conjugates and specific targets, such as Hpr1, a component of the THO complex. Here, we analyzed substrate specificity and ubiquitin selectivity of the Mex67 UBA domain. UBA-Mex67 is formed by three helices arranged in a classical UBA fold plus a fourth helix, H4. Deletion or mutation of helix H4 strengthens the interaction between UBA-Mex67 and ubiquitin, but it decreases its affinity for Hpr1. Interaction with Hpr1 is required for Mex67 UBA domain to bind polyubiquitin, possibly by inducing an H4-dependent conformational change. In vivo, deletion of helix H4 reduces cotranscriptional recruitment of Mex67 on activated genes, and it also shows an mRNA export defect. Based on these results, we propose that H4 functions as a molecular switch that coordinates the interaction of Mex67 with ubiquitin bound to specific substrates, defines the selectivity of the Mex67 UBA domain for polyubiquitin, and prevents its binding to nonspecific substrates.
Keywords		Active Transport, Cell Nucleus — Cell Nucleus/metabolism — Kinetics — Nuclear Magnetic Resonance, Biomolecular — Nuclear Proteins/chemistry/genetics/metabolism — Nucleocytoplasmic Transport Proteins/chemistry/genetics/metabolism — Protein Binding — Protein Structure, Secondary — Protein Structure, Tertiary — Protein Transport — RNA Transport — RNA, Messenger/genetics/metabolism — RNA-Binding Proteins/chemistry/genetics/metabolism — Saccharomyces cerevisiae/cytology/metabolism — Saccharomyces cerevisiae Proteins/chemistry/genetics/metabolism — Sequence Deletion — Solutions — Surface Plasmon Resonance — Transcription, Genetic — Ubiquitin/metabolism
Identifiers		DOI: 10.1091/mbc.E07-02-0153 PMID: 17475778
Full text		Article (Published version) (755 Kb) - Free access
Structures		Faculté des sciences / Section de biologie / Département de biologie cellulaire
Citation (ISO format)		HOBEIKA, Maria et al. Coordination of Hpr1 and ubiquitin binding by the UBA domain of the mRNA export factor Mex67. In: Molecular Biology of the Cell, 2007, vol. 18, n° 7, p. 2561-8. https://archive-ouverte.unige.ch/unige:13226