UNIGE document Scientific Article
previous document  unige:13122  next document
add to browser collection

Diversity of Raft-Like Domains in Late Endosomes

Parton, Robert G.
van der Goot, F. Gisou
Published in PloS one. 2007, vol. 2, no. 4, e391
Abstract Background Late endosomes, the last sorting station in the endocytic pathway before lysosomes, are pleiomorphic organelles composed of tubular elements as well as vesicular regions with a characteristic multivesicular appearance, which play a crucial role in intracellular trafficking. Here, we have investigated whether, in addition to these morphologically distinguishable regions, late endosomal membranes are additionally sub-compartmentalized into membrane microdomains. Methodology/Principal Findings Using sub-organellar fractionation techniques, both with and without detergents, combined with electron microscopy, we found that both the limiting membrane of the organel and the intraluminal vesicles contain raft-type membrane domains. Interestingly, these differentially localized domains vary in protein composition and physico-chemical properties. Conclusions/Significance In addition to the multivesicular organization, we find that late endosomes contain cholesterol rich microdomains both on their limiting membrane and their intraluminal vesicles that differ in composition and properties. Implications of these findings for late endosomal functions are discussed.
Full text
(ISO format)
SOBO, Komla et al. Diversity of Raft-Like Domains in Late Endosomes. In: PloS one, 2007, vol. 2, n° 4, p. e391. doi: 10.1371/journal.pone.0000391 https://archive-ouverte.unige.ch/unige:13122

549 hits



Deposited on : 2011-01-06

Export document
Format :
Citation style :