UNIGE document Scientific Article
previous document  unige:130902  next document
add to browser collection
Title

The rat hepatic leukemia factor (HLF) gene encodes two transcriptional activators with distinct circadian rhythms, tissue distributions and target preferences

Authors
Published in The EMBO journal. 1995, vol. 14, no. 17, p. 4307-4317
Abstract Hepatic leukemia factor (HLF) is a member of the PAR family of transcription regulatory proteins. We have characterized the rat HLF gene and studied its expression and activity. The rat HLF gene is transcribed from two alternative promoters, alpha and beta, with different circadian amplitudes and tissue specificities. The alpha RNA isoforms produce a 43 kDa protein, HLF43, abundant in brain, liver and kidney, like the previously described human HLF RNA. The beta RNA HLF isoforms use a CUG codon to initiate translation of a novel 36 kDa protein, HLF36, which is shorter at its N-terminus relative to the 43 kDa form. HLF36 is expressed uniquely in the liver, where it is the most abundant HLF protein. Surprisingly, the two proteins accumulate in the liver with different circadian amplitudes and have distinct liver-specific promoter preferences in transfection experiments. Thus, HLF43 stimulates transcription from the cholesterol 7 alpha-hydroxylase promoter much more efficiently than from the albumin promoter, while the converse is true for HLF36.
Keywords Amino Acid SequenceAnimalsBase SequenceBasic-Leucine Zipper Transcription FactorsBrain/metabolismCircadian RhythmCodonDNA Mutational AnalysisDNA PrimersDNAComplementaryDNA-Binding Proteins/biosynthesis/geneticsGene Expression RegulationHumansKidney/metabolismLiver/metabolismMolecular Sequence DataMutagenesisOligodeoxyribonucleotidesOrgan SpecificityPolymerase Chain ReactionPromoter RegionsGeneticProtein BiosynthesisRatsRecombinant Proteins/biosynthesisSequence DeletionSequence HomologyAmino AcidSpleen/metabolismTrans-Activators/biosynthesisTranscription Factors/biosynthesis/geneticsTranscriptionGeneticTransfection
Identifiers
PMID: 7556072
Full text
Article (Published version) (2.9 MB) - document accessible for UNIGE members only Limited access to UNIGE
Structures
Citation
(ISO format)
FALVEY, Eileen Elisabeth, FLEURY OLELA, Fabienne, SCHIBLER, Ulrich. The rat hepatic leukemia factor (HLF) gene encodes two transcriptional activators with distinct circadian rhythms, tissue distributions and target preferences. In: EMBO Journal, 1995, vol. 14, n° 17, p. 4307-4317. https://archive-ouverte.unige.ch/unige:130902

125 hits

0 download

Update

Deposited on : 2020-02-18

Export document
Format :
Citation style :