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Scientific article
Open access
English

IL-17E (IL-25) and IL-17A differentially affect the functions of human keratinocytes

Publication date2020
Abstract

Our group has recently shown that keratinocyte-derived IL-17E (IL-25), one of six members of the IL-17 family, is overexpressed in lesional psoriatic skin and is involved in its pathophysiology. We show here that IL-22 enhances IL-17E production in human keratinocytes and that these cells display a complete IL-17E receptor at their surface, which expression is further induced by IL-17A, indicating a potential autocrine effect of IL-17E. Therefore, we addressed the impact of IL-17E on the function of human primary keratinocytes. IL-17E promoted the proliferation of keratinocytes in 2D and 3D cultures and caused the concomitant up-regulation of differentiation-associated gene transcripts (e.g keratin 10), while their expression was either inhibited or not changed by IL-17A. Contrary to IL-17A, IL-17E was not involved in the induction of antimicrobial proteins. Time-lapse analysis of cell movement showed that IL-17E influences cell motility increasing both cell speed and displacement. This was associated with specific changes in the actin cytoskeleton organization and the cell-substrate adhesion. No such effects were observed upon IL-17A stimulation. In summary, we identified to our knowledge previously unreported effects of IL-17E clearly distinct from IL-17A, pointing towards an important role of IL-17E in the physiology and pathophysiology of the epidermis.

Keywords
  • IL-17A
  • IL-17E
  • IL-22
  • IL-25
  • differentiation
  • keratinocyte
  • migration
  • proliferation
  • psoriasis
  • wound healing
Citation (ISO format)
BOROWCZYK, Julia et al. IL-17E (IL-25) and IL-17A differentially affect the functions of human keratinocytes. In: The Journal of Investigative Dermatology, 2020. doi: 10.1016/j.jid.2019.12.013
Main files (1)
Article (Accepted version)
Identifiers
ISSN of the journal0022-202X
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119downloads

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Creation01/23/2020 3:01:00 PM
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