RIG-I and dsRNA-induced IFNbeta activation

Hausmann, Stéphane; Marq, Jean-Baptiste; Tapparel, Caroline; Kolakofsky, Daniel; Garcin, Dominique

doi:10.1371/journal.pone.0003965

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Title		RIG-I and dsRNA-induced IFNbeta activation
Authors		Hausmann, Stéphane Marq, Jean-Baptiste Tapparel, Caroline Kolakofsky, Daniel Garcin, Dominique
Published in		PloS one. 2008, vol. 3, no. 12, e3965
Abstract		Except for viruses that initiate RNA synthesis with a protein primer (e.g., picornaviruses), most RNA viruses initiate RNA synthesis with an NTP, and at least some of their viral (ppp)RNAs remain unblocked during the infection. Consistent with this, most viruses require RIG-I to mount an innate immune response, whereas picornaviruses require mda-5. We have examined a SeV infection whose ability to induce interferon depends on the generation of capped dsRNA (without free 5' tri-phosphate ends), and found that this infection as well requires RIG-I and not mda-5. We also provide evidence that RIG-I interacts with poly-I/C in vivo, and that heteropolymeric dsRNA and poly-I/C interact directly with RIG-I in vitro, but in different ways; i.e., poly-I/C has the unique ability to stimulate the helicase ATPase of RIG-I variants which lack the C-terminal regulatory domain.
Keywords		Animals — Binding Sites — Cells, Cultured — DEAD-box RNA Helicases/genetics/metabolism — Green Fluorescent Proteins/genetics/metabolism — Interferon-beta/immunology/metabolism — Mice — Poly I-C/genetics — Protein Structure, Tertiary — RNA, Double-Stranded/metabolism — Sendai virus/genetics/metabolism — Transfection — Viral Proteins/genetics/metabolism
Identifiers		DOI: 10.1371/journal.pone.0003965 PMID: 19115016
Full text		Article (Published version) (460 Kb) - Free access Other version: http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=19115016
Structures		Faculté de médecine / Section de médecine clinique / Département de médecine Faculté de médecine / Section de médecine fondamentale / Département de microbiologie et médecine moléculaire
Research group		Virologie moléculaire (275)
Citation (ISO format)		HAUSMANN, Stéphane et al. RIG-I and dsRNA-induced IFNbeta activation. In: PloS one, 2008, vol. 3, n° 12, p. e3965. doi: 10.1371/journal.pone.0003965 https://archive-ouverte.unige.ch/unige:1290