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unige:1290 
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RIG-I and dsRNA-induced IFNbeta activation |
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| Authors | ||
| Published in | PLOS ONE. 2008, vol. 3, no. 12, p. e3965 | |
| Abstract | Except for viruses that initiate RNA synthesis with a protein primer (e.g., picornaviruses), most RNA viruses initiate RNA synthesis with an NTP, and at least some of their viral (ppp)RNAs remain unblocked during the infection. Consistent with this, most viruses require RIG-I to mount an innate immune response, whereas picornaviruses require mda-5. We have examined a SeV infection whose ability to induce interferon depends on the generation of capped dsRNA (without free 5' tri-phosphate ends), and found that this infection as well requires RIG-I and not mda-5. We also provide evidence that RIG-I interacts with poly-I/C in vivo, and that heteropolymeric dsRNA and poly-I/C interact directly with RIG-I in vitro, but in different ways; i.e., poly-I/C has the unique ability to stimulate the helicase ATPase of RIG-I variants which lack the C-terminal regulatory domain. | |
| Keywords | Animals — Binding Sites — Cells, Cultured — DEAD-box RNA Helicases/genetics/metabolism — Green Fluorescent Proteins/genetics/metabolism — Interferon-beta/immunology/metabolism — Mice — Poly I-C/genetics — Protein Structure, Tertiary — RNA, Double-Stranded/metabolism — Sendai virus/genetics/metabolism — Transfection — Viral Proteins/genetics/metabolism | |
| Identifiers | PMID: 19115016 | |
| Full text |
Article (Author postprint) (460 Kb) - Free access Other version: http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=19115016 |
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| Structures | ||
| Research group | Virologie moléculaire (275) | |
| Citation (ISO format) | HAUSMANN, Stéphane et al. RIG-I and dsRNA-induced IFNbeta activation. In: PLOS ONE, 2008, vol. 3, n° 12, p. e3965. https://archive-ouverte.unige.ch/unige:1290 |
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