Scientific article
Open access

The role of GRIP1 and ephrin B3 in blood pressure control and vascular smooth muscle cell contractility

Published inScientific Reports, vol. 6, 38976
Publication date2016

Several erythropoietin-producing hepatocellular receptor B family (EPHB) and their ligands, ephrinBs (EFNBs), are involved in blood pressure regulation in animal models. We selected 528 single nucleotide polymorphisms (SNPs) within the genes of EPHB6, EFNB2, EFNB3 and GRIP1 in the EPH/EFN signalling system to query the International Blood Pressure Consortium dataset. A SNP within the glutamate receptor interacting protein 1 (GRIP1) gene presented a p-value of 0.000389, approaching the critical p-value of 0.000302, for association with diastolic blood pressure of 60,396 individuals. According to echocardiography, we found that Efnb3 gene knockout mice showed enhanced constriction in the carotid arteries. In vitro studies revealed that in mouse vascular smooth muscle cells, siRNA knockdown of GRIP1, which is in the EFNB3 reverse signalling pathway, resulted in increased contractility of these cells. These data suggest that molecules in the EPHB/EFNB signalling pathways, specifically EFNB3 and GRIP1, are involved blood pressure regulation.

  • Animals
  • Blood Pressure
  • Carrier Proteins/genetics/metabolism
  • Ephrin-B3/genetics/metabolism
  • Humans
  • Mice
  • Inbred C57BL
  • Mice
  • Knockout
  • Muscle Contraction
  • Muscle
  • Smooth
  • Vascular/metabolism
  • Nerve Tissue Proteins/genetics/metabolism
  • Polymorphism
  • Single Nucleotide
  • Signal Transduction
Affiliation Not a UNIGE publication
Citation (ISO format)
WANG, Yujia et al. The role of GRIP1 and ephrin B3 in blood pressure control and vascular smooth muscle cell contractility. In: Scientific Reports, 2016, vol. 6, p. 38976. doi: 10.1038/srep38976
Main files (1)
Article (Published version)
Secondary files (1)
ISSN of the journal2045-2322

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