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Scientific article
English

A comparison of Sensititre™ Anaerobe MIC plate with ATB ANA® test for the routine susceptibility testing of common anaerobe pathogens

Published inEuropean Journal of Clinical Microbiology & Infectious Diseases, vol. 37, no. 12, p. 2279-2284
Publication date2018
Abstract

The accuracy of the Thermo Scientific™ Sensititre™ Anaerobe MIC plate was assessed against the ATB ANA® test (bioMérieux) on 56 clinically relevant anaerobic strains collected at Geneva University Hospitals. The overall categorical agreement between both methods reached 95%. The Sensititre™ Anaerobe MIC plate had excellent accuracy for most antibiotics tested. When the Sensititre™ Anaerobe MIC plate disagreed with ATB ANA® test, the gradient strip method resolved the antimicrobial susceptibility categories of all the antibiotics tested, except for piperacillin, piperacillin-tazobactam, and penicillin, in favor of the Sensititre™ Anaerobe MIC plate (58% [21 out of 36]). Several very major errors were observed for piperacillin (12.5% [7 out of 56]), piperacillin-tazobactam (12.5% [7 out of 56]), and penicillin (2% [1 out of 56]). The gradient strip method revealed that the categorical differences for piperacillin, piperacillin-tazobactam, and penicillin were at least partly explained by heterogeneity in resistance expression. The Sensititre™ Anaerobe MIC plate offers therefore a useful alternative to the ATB ANA® test for the routine antimicrobial susceptibility testing of anaerobes in clinical microbiology laboratories.

Keywords
  • Anti-Bacterial Agents/pharmacology
  • Bacteria
  • Anaerobic/drug effects
  • Cross Infection/microbiology
  • Gram-Negative Bacteria/drug effects
  • Gram-Positive Bacteria/drug effects
  • Microbial Sensitivity Tests/instrumentation/methods
  • Penicillins/pharmacology
  • Piperacillin/pharmacology
  • Switzerland
Citation (ISO format)
CHERKAOUI, Abdessalam et al. A comparison of Sensititre™ Anaerobe MIC plate with ATB ANA® test for the routine susceptibility testing of common anaerobe pathogens. In: European Journal of Clinical Microbiology & Infectious Diseases, 2018, vol. 37, n° 12, p. 2279–2284. doi: 10.1007/s10096-018-3369-5
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Article (Published version)
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ISSN of the journal0934-9723
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