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A 61,000-Dalton Truncated Large T-Antigen is Uniformly Expressed in Hamster Cells Transformed by Polyomavirus

Rey-Bellet, Véronique
Published in Journal of Virology. 1984, vol. 50, no. 2, p. 587-597
Abstract Various polyomavirus-transformed hamster cell lines derived from tumors or from infected hamster cell cultures synthesized polyoma middle and small tumor (T)-antigens but no full-size large T-antigen. Instead, all cell lines produced the same or similar polyoma T-antigen-related proteins of ca. 61 kilodaltons (kDal). Like large T-antigen synthesized in lytically infected mouse cells, the 61-kDal proteins were phosphoproteins showing electrophoretic and charge heterogeneities. Chromatographic analysis of the methionine-containing tryptic peptides indicated that the 61-kDal proteins were truncated forms of large T-antigen comprising amino acid residues 1 to 485 (+/- 25). Analysis of viral DNA present in hamster chromosomal DNA of three independently isolated cell lines confirmed that synthesis of the 61-kDal proteins was due to a discontinuity in the large T-antigen coding sequence, most likely located between 7 and 8.9 map units on the polyoma DNA map. The three cell lines yielded essentially the same patterns of viral DNA-containing restriction enzyme fragments, suggesting that insertion of viral DNA into the hamster chromosomes took place at closely similar sites.
Keywords AnimalsPolyomavirus transforming antigensNeoplastic cell transformationCultured cellsCricetinaeDNA/isolation & purificationDNA Restriction EnzymesKidney/enzymologyMesocricetusMiceMolecular WeightPeptide Fragments/analysisPolyomavirus/enzymology/geneticsProtein Kinases/geneticsViral Proteins/genetics/isolation & purification
PMID: 6323758
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Swiss National Science Foundation: 3.474.79 et 3.012.81
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REY-BELLET, Véronique, TURLER, Hans. A 61,000-Dalton Truncated Large T-Antigen is Uniformly Expressed in Hamster Cells Transformed by Polyomavirus. In: Journal of Virology, 1984, vol. 50, n° 2, p. 587-597. https://archive-ouverte.unige.ch/unige:128116

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Deposited on : 2019-12-20

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