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Scientific article
Open access
English

Deletions Fusing the i and lac Regions of the Chromosome in E. coli: Isolation and Mapping

Published inMolecular and General Genetics, vol. 131, no. 2, p. 137-146
Publication date1974
Abstract

Three hundred and fifty deletions ending within the i gene (which codes for the lac repressor) at one terminus and within the z gene at the other terminus have been isolated. Twenty-nine of these have been mapped in detail and have endpoints which are distributed throughout the i gene. Two deletions which remove only the very end of the i gene still permit the synthesis of altered repressor molecules retaining some properties of the wildtype lac repressor. In strains carrying these deletions, the synthesis of lac permease is under the control of the i promoter. An additional 40 deletions have been found which apparently fuse the lac permease to an untranslated portion of the terminus of the i-message.

Keywords
  • Endpoint
  • Repressor molecule
  • Untranslated portion
Funding
  • Autre - Deutsche Forschungsgemeinschaft SFB74
  • Swiss National Science Foundation - 3.800.72
Citation (ISO format)
GHO, Djim, MILLER, Jeffrey H. Deletions Fusing the <i>i</i> and <i>lac</i> Regions of the Chromosome in <i>E. coli</i>: Isolation and Mapping. In: Molecular and General Genetics, 1974, vol. 131, n° 2, p. 137–146. doi: 10.1007/BF00266149
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Article (Published version)
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Identifiers
ISSN of the journal0026-8925
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173downloads

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