Scientific article
English

Cloning and expression of the phage Mu A gene

Published inGene, vol. 28, no. 1, p. 65-72
Publication date1984
Abstract

We have cloned the phage Mu A gene, with and without the gene ner, under the control of the PL promoter of phage λ in a multicopy plasmid vector. We demonstrate that plasmid-carrying cells are able to support growth of superinfecting Mu A am phages in a temperature-dependent fashion in a host strain carrying a defective λ prophage which specifies the cI857-coded λ repressor. In addition, we show that the presence of the ner gene reduces the efficiency of plating of the superinfecting phage. Analysis of proteins specified by the cloned Mu fragments indicates that two proteins, 70 and 33 kDal, are synthesized. The level of synthesis, compared to that of the vector-encoded ß-lactamase, was found to increase with temperature. This indicates that their transcription is driven by the PL promoter. The MPr of the 70-kDal protein is identical to that previously observed for pA.

Keywords
  • Recombinant DNA
  • Plasmid pPLc236::nerA
  • Plasmid pPLc236::A
  • Complementation assay
  • Protein synthesis in minicells
  • Phage lambda vector
  • PL promoter
  • Repressor
Funding
  • Swiss National Science Foundation - 3.169.81
Citation (ISO format)
ROULET, Emmanuelle, ALLET, Bernard, CHANDLER, Michael. Cloning and expression of the phage Mu A gene. In: Gene, 1984, vol. 28, n° 1, p. 65–72. doi: 10.1016/0378-1119(84)90088-X
Main files (1)
Article (Published version)
accessLevelRestricted
Identifiers
ISSN of the journal0378-1119
222views
0downloads

Technical informations

Creation12/10/2019 12:44:00 PM
First validation12/10/2019 12:44:00 PM
Update time03/15/2023 6:34:35 PM
Status update03/15/2023 6:34:35 PM
Last indexation10/31/2024 5:14:13 PM
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack