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Title

DNAs of simian virus 40 and polyoma direct the synthesis of viral tumor antigens and capsid proteins in Xenopus oocytes
Authors
Rungger, Duri
Turler, Hans
Published in Proceedings of the National Academy of Sciences. 1978, vol. 75, no. 12, p. 6073-6077
Abstract Purified simian virus 40 and polyoma DNAs injected into nuclei of Xenopus oocytes were transcribed and subsequently translated into virus-specific tumor antigens and capsid proteins. Simian virus 40 large and small tumor antigens synthesized in the oocytes were indistinguishable, by gel electrophoresis and [35S]methionine-labeled tryptic peptide mapping, from the corresponding polypeptides synthesized in CV-1 African green monkey cells. The synthesis of large simian virus 40 tumor antigen implies the correct splicing of its mRNA, which is complementary to nonadjacent nucleotide sequences in the early region of the viral genome. Polyoma DNA directed synthesis of two polyoma tumor antigen polypeptides, 57,000 Mr and small tumor antigen, and of the main capsid protein.
Keywords Microinjection — Coupled transcription and translation — Immunoprecipitation — mRNA splicing
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Article (Published version) (1.6 MB) - public document Free access
Other version: http://www.pnas.org/cgi/doi/10.1073/pnas.75.12.6073
Structures
Faculté des sciences / Section de biologie / Département de biologie moléculaire
Citation
(ISO format) 		RUNGGER, Duri, TURLER, Hans. DNAs of simian virus 40 and polyoma direct the synthesis of viral tumor antigens and capsid proteins in Xenopus oocytes. In: Proceedings of the National Academy of Sciences, 1978, vol. 75, n° 12, p. 6073-6077. doi: 10.1073/pnas.75.12.6073 https://archive-ouverte.unige.ch/unige:127555

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Deposited on : 2019-12-06

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