UNIGE document Scientific Article
previous document  unige:127239  next document
add to browser collection

3D Cellular Architecture Affects MicroRNA and Protein Cargo of Extracellular Vesicles

Rocha, Sara
Carvalho, Joana
Oliveira, Patrícia
Voglstaetter, Maren
Thomsen, Andreas R
Khanduri, Richa
show hidden authors show all authors [1 - 12]
Published in Advanced Science. 2019, vol. 6, no. 4, p. 1800948
Abstract The success of malignant tumors is conditioned by the intercellular communication between tumor cells and their microenvironment, with extracellular vesicles (EVs) acting as main mediators. While the value of 3D conditions to study tumor cells is well established, the impact of cellular architecture on EV content and function is not investigated yet. Here, a recently developed 3D cell culture microwell array is adapted for EV production and a comprehensive comparative analysis of biochemical features, RNA and proteomic profiles of EVs secreted by 2D vs 3D cultures of gastric cancer cells, is performed. 3D cultures are significantly more efficient in producing EVs than 2D cultures. Global upregulation of microRNAs and downregulation of proteins in 3D are observed, indicating their dynamic coregulation in response to cellular architecture, with the ADP-ribosylation factor 6 signaling pathway significantly downregulated in 3D EVs. The data strengthen the biological relevance of cellular architecture for production and cargo of EVs.
PMID: 30828519
Full text
Article (Published version) (4.6 MB) - public document Free access
Research group Groupe de Protéomique biomédicale (635)
(ISO format)
ROCHA, Sara et al. 3D Cellular Architecture Affects MicroRNA and Protein Cargo of Extracellular Vesicles. In: Advanced Science, 2019, vol. 6, n° 4, p. 1800948. doi: 10.1002/advs.201800948 https://archive-ouverte.unige.ch/unige:127239

141 hits



Deposited on : 2019-12-02

Export document
Format :
Citation style :